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Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos
(2013-10-01)
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...
Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos
(2013-10-01)
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...
Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
(Pontificia Universidad Católica de Valparaíso, 2011)
New visible and selective DNA staining method in gels with tetrazolium salts
(Academic Press Inc., 2017)
© 2016 Elsevier Inc.DNA staining in gels has historically been carried out using silver staining and fluorescent dyes like ethidium bromide and SYBR Green I (SGI). Using fluorescent dyes allows recovery of the analyte, but ...
Implementación y evaluación del ensayo amplificación isotérmica mediada por un lazo (lamp)para detección de brucella abortus en muestras biológicas de bovinos
(2017)
Bovine brucellosis is an infectious-transmissible disease caused by Brucella abortus, which is characterized by mainly affecting the reproductive tract of adult females. This disease causes great impacts in the economic ...
Plasmodium vivax: parasitemia determination by real-time quantitative PCR in Aotus monkeys
Plasmodium vivax and Plasmodium falciparum are the two prevalent human malaria species. A Colombian P. vivax wild strain has been adapted in Aotus nancymaae monkeys for use in further biological and immunological studies. ...
Estandarización de un método fluorométrico para la determinación de la densidad parasitaria por Plasmodium en modelos farmacológicos de malaria in vivo e in vitro
(2015-12-09)
La eficacia in vivo e in vitro de potenciales antimaláricos generalmente es evaluada mediante la determinación microscópica directa de la parasitemia de Plasmodium en extendidos de sangre teñidos con Giemsa. Este proceso ...
Adaptación y optimización de un método de lectura por fluorometría en el modelo farmacológico in vitro de cultivo de Plasmodium falciparum
(Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Farmacia, 2016-01-01)
El modelo farmacológico de cultivo in vitro de P. falciparum es crucial en el tamizaje inicial de sustancias o extractos de plantas con posible actividad antiplasmodial. La densidad parasitaria puede determinarse mediante ...