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Sperm sexing mediated by magnetic nanoparticles in donkeys, a preliminary in vitro study
(Elsevier Science Inc, 2018-06)
Preselection of sex before conception has been one of the objectives pursued by scientists and breeders for many years. The donkey milk industry has shown a greater commercial interest, since jennies’ demand is increasing ...
Effect of different mini-volume colloid centrifugation configurations on flow cytometrically sorted sperm recovery efficiency and quality using a computer-assisted semen analyzer
(Wiley Blackwell Publishing, Inc, 2018-02)
Straws of sex-sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 106 sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of ...
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis
(2018-11-01)
The identification of distinct proteins present on the membrane of spermatozoa with X and Y chromosomes allows the development of immuno-sexing techniques. The aim of this study, therefore, was to use mass spectrometry to ...
Improved bovine in vitro embryo production with sexed and unsexed sperm selected by chemotaxis
(Elsevier Science Inc, 2018-12)
Assisted reproductive techniques (ART) have been widely used in farm animals in the last decades. Sexed cryopreserved spermatozoa, ovum pick up, in vitro embryo production and transfer constitute the ART that have ...
Sperm sexing with density gradient centrifugation in dogs
(Elsevier B.V., 2018-12-01)
Sexed sperm in dogs is of interest because of being polytocous, and as a result, the greatest number of offspring of the same sex can improve the market, although few studies assessing sperm sexing have been performed in ...
Isolate((R)) and Optiprep((R)) minigradients as alternatives for sperm selection in bovine in vitro embryo production
(Agricultural Inst Canada, 2014-03-01)
The objective of this study was to evaluate alternatives in small volumes to conventional gradient of Percoll((R)) on semen quality, in vitro embryo production, sex ratio and embryo survival after vitrification. Thawed ...
Flow cytometry sex sorting affects bull sperm longevity and compromises their capacity to bind to oviductal cells
(Elsevier B.V., 2018-01-01)
This study assessed the effect of flow cytometry sexing on sperm longevity and the capacity of sperm to bind to oviductal cells. Each ejaculate from four bulls was divided into two fractions: the first was immediately ...
Sperm pretreatment with heparin and L-glutathione, sex-sorting, and double cryopreservation to improve intracytoplasmic sperm injection in bovine
(Elsevier Science Inc, 2017-04)
In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with ...
Different sperm allocation strategies in two populations of the semiterrestrial crab Neohelice granulata (Brachyura, Grapsoidea, Varunidae)
(Wiley Blackwell Publishing, Inc, 2016-08)
Sperm reserves are costly to produce and so the development of different sperm allocation strategies may be advantageous. Knowledge of sperm allocation strategies is important from an evolutionary perspective because they ...
Testes Mass, but Not Sperm Length, Increases with Higher Levels of Polyandry in an Ancient Sex Model
(Public Library of Science, 2014-04)
There is strong evidence that polyandrous taxa have evolved relatively larger testes than monogamous relatives. Sperm size may either increase or decrease across species with the risk or intensity of sperm competition. ...