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Seasonal variations in the composition of ram seminal plasma and its effect on frozen-thawed ram sperm
(Elsevier Science Inc, 2008-03)
It has been proposed that seminal plasma (SP) in the extender or in post-thaw media can prevent and revert cold-shock damage in cryopreserved ram sperm; however, this was dependent on season. We evaluated sperm parameters ...
Simultaneous analysis of five antidepressant drugs using direct injection of biofluids in a capillary restricted-access media-liquid chromatography-tandem mass spectrometry system
(ELSEVIER SCIENCE BV, 2008)
Direct analysis, with minimal sample pretreatment, of antidepressant drugs, fluoxetine, imipramine, desipramine, amitriptyline, and nortriptyline in biofluids was developed with a total run time of 8 min. The setup consists ...
Components of the extender and seminal plasma can alter the response of ram spermatozoa to cryopreservation
(Ediciones Taurus, 2019-12)
The cryopreservation of sheep semen represents a challenge to increase pregnancy rates in cervical artificial insemination programs. Decreased sensitivity at low temperatures could counteract certain detrimental effects ...
Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology
(Elsevier Science, 2016-01)
During the last decades fundamental and applied aspects of mammalian ram sperm cryopreservation have been increasingly explored by scientists and biotechnologists. Many works report modifications in the composition of the ...
Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology
(Elsevier, 2016-01)
During the last decades fundamental and applied aspects of mammalian ram sperm cryopreservation have been increasingly explored by scientists and biotechnologists. Many works report modifications in the composition of the ...
Determinação de estatinas em plasma humano empregando cromatografia líquida com colunas de meio de acesso restrito
(Universidade Federal de Minas GeraisUFMG, 2013-03-01)
This study presents the preparation of a Restricted Access Media (RAM) column with immobilized bovine serum albumin (BSA) by crosslinking with glutaraldehyde. The homemade RAM column was compared with commercial alkyl-diol ...
Desenvolvimento de um método para quantificação simultânea de sulfametoxazol e trimetoprima em ovos de galinha utilizando a cromatografia liquida de alta eficiência bidimensional
(Universidade Federal de São CarlosBRUFSCarPrograma de Pós-Graduação em Química - PPGQ, 2007-04-13)
This work reports the investigation of a series of bovine serum albumin (BSA) restricted access media columns (RAM) for on-line egg samples deproteinization. High exclusion percentage of egg s proteins were obtained with ...
On-line solid-phase extraction of pharmaceutical compounds from wastewater treatment plant samples using restricted access media in column-switching liquid chromatography-tandem mass spectrometry
(2021-08-15)
An on-line solid phase extraction using a lab-made restricted access media (RAM) was developed as sample preparation procedure for determination of the pharmaceutical compounds caffeine (CAF), carbamazepine (CBZ), norfloxacin ...
Métodos para a determinação enantiomérica dos fármacos omeprazol, lansoprazol e pantoprazol em águas residuais e de estuário
(Universidade Federal de São CarlosBRUFSCarPrograma de Pós-Graduação em Química - PPGQ, 2009-04-17)
The use of restricted access media columns for direct injection of samples chemically complex is consolidated, but not for environmental samples. This work describes the conditions for exclusion of aquatic fulvic and humic ...
Quantificação dos metabólitos do albendazol em plasma bovino por cromatografia líquida quiral de alta eficiência, com injeção direta de amostras
(Universidade Federal de São CarlosBRUFSCarPrograma de Pós-Graduação em Química - PPGQ, 2007-08-17)
This work presents the development and validation of a sensitive and enantioselective multidimensional high performance liquid chromatography (HPLC) method for determination of albendazole metabolites: albendazole sulphoxide, ...