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Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding
(Asociación Argentina de Microbiología, 2016-01)
Bacillus anthracis protective antigen (PA) is a well known and relevant immunogenicprotein that is the basis for both anthrax vaccines and diagnostic methods. Properly foldedantigenic PA is necessary for these applications. ...
Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli
(Pontificia Universidad Católica de Valparaíso, 2011)
Heterologous expression, purification and refolding of an anti-listerial peptide produced by Pediococcus acidilactici K7
(Pontificia Universidad Católica de Valparaíso, 2007)
Production of the refolded oligopeptide-binding protein (OppA) encoded by the citrus pathogen Xanthomonas axonopodis pv. citri
(FUNPEC-EDITORA, 2008)
The oligopeptide-binding protein, OppA, binds and ushers oligopeptide substrates to the membrane-associated oligopeptide permease (Opp), a multi-component ABC-type transporter involved in the uptake of oligopeptides expressed ...
Investigation on solubilization protocols in the refolding of the thioredoxin TsnC from Xylella fastidiosa by high hydrostatic pressure approach
(ElsevierMaryland Heights, 2015-02)
The lack of efficient refolding methodologies must be overcome to take full advantage of the fact that bacteria express high levels of aggregated recombinant proteins. High hydrostatic pressure (HHP) impairs intermolecular ...
Protein refolding at high pressure: Optimization using eGFP as a model
(Elsevier Sci LtdOxfordInglaterra, 2011)
Effect of pressure on refolding of recombinant pentameric cholera toxin B
(Elsevier Science PublishersAmsterdam, 2014-03-10)
The production of recombinant proteins is an essential tool for the expansion of modern biological research and biotechnology. The expression of heterologous proteins in Escherichia coli often results in an incomplete ...
Refolding and purification of bothropstoxin-1, a Lys49 - Phospholipase A(2) homologue, expressed as inclusion bodies in Escherichia coli
(Academic Press Inc., 2001-02-01)
Hydrolysis of phospholipids by Group II phospholipase A(2) enzymes involves a nucleophilic attack on the sn-2 ester bond by the His48 residue and stabilization of the reaction intermediate by a Ca2+ ion cofactor bound to ...
Refolding and purification of bothropstoxin-1, a Lys49 - Phospholipase A(2) homologue, expressed as inclusion bodies in Escherichia coli
(Academic Press Inc., 2001-02-01)
Hydrolysis of phospholipids by Group II phospholipase A(2) enzymes involves a nucleophilic attack on the sn-2 ester bond by the His48 residue and stabilization of the reaction intermediate by a Ca2+ ion cofactor bound to ...
Muscular hypertrophy and growth‐promoting effects in juvenile pejerrey (Odontesthes bonariensis) after oral administration of recombinant homologous growth hormone obtained by a highly efficient refolding process
(Wiley Blackwell Publishing, Inc, 2011-05)
Growth hormone (GH) can be orally administrated to fish in order to increase growth rates. Fish growth is characterized by the hyperplasia and hypertrophy of muscle fibre throughout adult life. In this respect, GH could ...