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Evaluation of Quantitative RT-PCR Using Nonamplified and Amplified RNA
(LIPPINCOTT WILLIAMS & WILKINS, 2010)
Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is a standard assay in molecular medicine for gene expression analysis. Samples from incisional/needle biopsies, laser-microdissected tumor cells and ...
A Sigmoidal Model for the Interpretation of Quantitative PCR (QPCR) Experiments
(Universidad Militar Nueva Granada, 2016)
Desenvolvimento e validação de uma reação em cadeia da polimerase quantitativa (qPCR) para diagnóstico da zoonose pseudovaríola bovina
(Universidade Federal de Minas GeraisBrasilFARMACIA - FACULDADE DE FARMACIAPrograma de Pós-Graduação em Análises Clínicas e ToxicológicasUFMG, 2019-03-08)
The pseudocowpox is a zoonosis caused by the pseudocowpox vírus (PCPV), classified within the genus Poxvirinae, Poxviridae family. The pseudocowpox affects mainly cows, also reaching suckling calves. In humans, it is a ...
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
(Elsevier, 2020-12-24)
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders ...
Evaluation of detachment methods for the enumeration ofBacteroides fragilis in sediments via propidium monoazide quantitative PCR, in comparison with Enterococcus faecalis and Escherichia coli
(Wiley, 2014-08)
Aims
The aim was to develop an optimized detachment method for separating Bacteroidales from sediments to allow enumeration via PMA-qPCR. The effectiveness of four different detachment treatments in removing Bacteroides ...
Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with a bovine rotavirus VP6 experimental vaccine
(Elsevier, 2013-04)
In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing ...
Quantitative real-time PCR method to detect changes in specific transcript and total RNA amounts
(Pontificia Universidad Católica de Valparaíso, 2004)
Development of a New Genus-Specific Quantitative Real-Time PCR Assay for the Diagnosis of Scrub Typhus in South America
(2022)
Scrub typhus is a potentially severe rickettsiosis, caused by Orientia tsutsugamushi in the Asia-Pacific region. Recently, however, two distinct pathogens, “Candidatus Orientia chuto” and “Candidatus Orientia chiloensis”, ...