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Characterization, subsite mapping and N-terminal sequence of miliin, a serine-protease isolated from the latex of Euphorbia milii
(Elsevier B.V., 2013-04-01)
Miliin is a serine protease purified from the latex of Euphorbia milii. This work reports the effect of pH and temperature on the catalytic activity of miliin, using fluorescence resonance energy transfer (FRET) substrates. ...
Variability and resistance mutations in the hepatitis C virus NS3 protease in patients not treated with protease inhibitors
(Instituto Oswaldo Cruz, Ministério da Saúde, 2013-02-01)
The goal of treatment of chronic hepatitis C is to achieve a sustained virological response, which is defined as exhibiting undetectable hepatitis C virus (HCV) RNA levels in serum following therapy for at least six months. ...
Variability and resistance mutations in the hepatitis C virus NS3 protease in patients not treated with protease inhibitors
(Instituto Oswaldo Cruz, Ministério da Saúde, 2013-02-01)
The goal of treatment of chronic hepatitis C is to achieve a sustained virological response, which is defined as exhibiting undetectable hepatitis C virus (HCV) RNA levels in serum following therapy for at least six months. ...
De novo assembly of Vriesea carinata leaf transcriptome to identify candidate cysteine-proteases
(Elsevier, 2019)
Vriesea carinata is an endemic bromeliad from the Brazilian Atlantic Forest. It has trichome and tank system in their leaves which allows to absorb water and nutrients. It belongs to Bromeliaceae family, which includes ...
Purification and characterization of a new alkaline serine protease from the thermophilic fungus Myceliophthora sp.
(Elsevier B.V., 2011-11-01)
This work reports the purification of a novel alkaline protease enzyme from a putative new thermophilic fungus Myceliophthora sp. The molecular weight of the enzyme was determined as 28.2 kDa by using MALDI-TOF MS and it ...
Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
(2020-12-01)
Enzymes from cold-adapted microorganisms are of high interest to industries due to their high activity at low and mild temperatures, which makes them suitable for their use in several processes that either require a supply ...
Purification and properties of an alkaline protease of Aspergillus clavatus
(Springer, 2007-02-01)
An extracellular alkaline serine protease has been purified from a strain of Aspergillus clavatus, to apparent homogeneity, by ammonium sulfate precipitation and chromatography on Sephadex G-75. Its molar mass, estimated ...
Purification and properties of an alkaline protease of Aspergillus clavatus
(Springer, 2007-02-01)
An extracellular alkaline serine protease has been purified from a strain of Aspergillus clavatus, to apparent homogeneity, by ammonium sulfate precipitation and chromatography on Sephadex G-75. Its molar mass, estimated ...