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Gene Mutations in Esophageal Mucosa of Chagas Disease Patients
(Int Inst Anticancer Research, 2009-04-01)
Background: Chagas disease patients with longstanding megaesophagus have a significantly increased risk for esophageal carcinoma. Materials and Methods: PCR-SSCP analysis and DNA sequencing of esophageal mucosa from Chagas ...
Diversity of cry genes and genetic characterization of Bacillus thuringiensis isolated from Brazil
(2004-08-01)
Two hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes by PCR with primers specific to different cry and cyt genes. Among these isolates, 95 were ...
Diversity of cry genes and genetic characterization of Bacillus thuringiensis isolated from Brazil
(2004-08-01)
Two hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes by PCR with primers specific to different cry and cyt genes. Among these isolates, 95 were ...
Identification and frequency of transposable elements in Eucalyptus
(2005-12-01)
Transposable elements (TE) are major components of eukaryotic genomes and involved in cell regulation and organism evolution. We have analyzed 123,889 expressed sequence tags of the Eucalyptus Genome Project database and ...
Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of canine ehrlichiosis
(Colégio Brasileiro de Parasitologia Veterinária, 2010-06-01)
O objetivo deste estudo foi aperfeiçoar um ensaio de PCR que amplificasse um fragmento de 843 pares de bases do gene p28 da Ehrlichia canis e compará-lo com outros dois métodos de PCR utilizados para amplificar partes do ...
Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of canine ehrlichiosis
(Colégio Brasileiro de Parasitologia Veterinária, 2010-06-01)
O objetivo deste estudo foi aperfeiçoar um ensaio de PCR que amplificasse um fragmento de 843 pares de bases do gene p28 da Ehrlichia canis e compará-lo com outros dois métodos de PCR utilizados para amplificar partes do ...
Mapping MHC Genes in River Buffalo
(Karger, 2008-01-01)
The major histocompatibility complex (MHC) contains a set of genes necessary for antigen presentation in the immune system. This gene dense and polymorphic region of the mammalian genome is of considerable interest due to ...
Synthetic and natural polycations for gene therapy: State of the art and new perspectives
(Bentham Science Publ Ltd, 2006-02-01)
Currently, the major drawback of gene therapy is the gene transfection rate. The two main types of vectors that. are used in gene therapy are based on viral or non-viral gene delivery systems. There are several non-viral ...