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Human recombinant membrane-bound aminopeptidase P: production of a soluble form and characterization using novel, internally quenched fluorescent substrates
(Portland Press Ltd, 2005-01-15)
APP (aminopeptidase P) has the unique ability to cleave the N-terminal amino acid residue from peptides exhibiting a proline at P-1'. Despite its putative involvement in the processing of bioactive peptides, among them the ...
S3 to S3 ' subsite specificity of recombinant human cathepsin K and development of selective internally quenched fluorescent substrates
(Portland Press, 2003-08-01)
We have systematically examined the S3 to S3' subsite substrate specificity requirements of cathepsin K using internally quenched fluorescent peptides derived from the lead sequence Abz-KLRFSKQ-EDDnp [where Abz is ...
Synthesis and hydrolysis by cysteine and serine proteases of short internally quenched fluorogenic peptides
(Academic Press Inc, 2001-06-01)
We developed sensitive substrates for cysteine proteases and specific substrates for serine proteases based on short internally quenched fluorescent peptides, Abz-F-R-X-EDDnp, where Abz (ortho-aminobenzoic acid) is the ...
Specificity of human tissue kallikrein towards substrates containing Phe-Phe pair of amino acids
(Portland Press, 1999-04-15)
We have explored in detail the determinants of specificity for the hydrolysis by human tissue kallikrein (hK(1)) of substrates containing the Phe-Phe amino acid pair, after which hK(1) cleaves kallistatin (human ...
Differences in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins
(Portland Press, 2004-06-15)
The kininogenase activities of mouse (mK1), rat (rK1) and human (hK1) tissue kallikrems were assayed with the bradykinin-containing synthetic peptides NH2-MTEMARRPPGFSPFRSVTVQ-NH2 (where Abz stands for o-aminobenzoyl) and ...
Subsite specificity (S3, S2, S1 ', S2 ' and S3 ') of oligopeptidase B from Trypanosoma cruzi and Trypanosoma brucei using fluorescent quenched peptides: comparative study and identification of specific carboxypeptidase activity
(Portland Press Ltd, 2003-08-01)
We characterized the extended substrate binding site of recombinant oligopeptidase B enzymes from Trypanosoma cruzi (Tc-OP) and Trypanosoma brucei (Tb-OP), evaluating the specificity of their S3, S2, S1', S2' and S3' ...
Analysis of peptidase activities of a cathepsin B-like (TcoCBc1) from Trypanosoma congolense
(Elsevier B.V., 2014-07-01)
The substrate specificity of TcoCBc1 was evaluated using two internally quenched fluorescent peptide libraries with randomized sequences designed to detect carboxydipeptidase (Abz-GXXZXK(Dnp)-OH) and endopeptidase ...
Subsite substrate specificity of midgut insect chymotrypsins
(PERGAMON-ELSEVIER SCIENCE LTD, 2008)
Insect chymotrypsins are distinctively sensitive to plant protein inhibitors, suggesting that they differ in subsite architecture and hence in substrate specificities. Purified digestive chymotrypsins from insects of three ...
Substrate specificity characterization of recombinant metallo oligo-peptidases thimet oligopeptidase and neurolysin
(Amer Chemical Soc, 2001-04-10)
We report a systematic and detailed analysis of recombinant neurolysin (EC 3.4.24.16) specificity in parallel with thimet oligopeptidase (TOP, EC 3.4.24.15) using Bk sequence and its C- and N-terminal extensions as in human ...
Structural features that make oligopeptides susceptible substrates for hydrolysis by recombinant thimet oligopeptidase
(Portland Press, 1997-06-01)
A systematic analysis of the peptide sequences and lengths of several homologues of bioactive peptides and of a number of quenched-fluorescence (qf) opioid- and bradykinin-related peptides was performed to determine the ...