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Purification of rabies virus glycoprotein produced in Drosophila melanogaster S2 cells: An efficient immunoaffinity method
(2020-01-01)
Most rabies vaccines are based on inactivated virus, which production process demands a high level of biosafety structures. In the past decades, recombinant rabies virus glycoprotein (RVGP) produced in several expression ...
DNA removal from a purification process of recombinant hepatitis B surface antigen
(Pontificia Universidad Católica de Valparaíso, 2007)
Generation and evaluation of a monoclonal antibody anti-pili k99 from enteropathogenic escherichia coli
(UNIV AUSTRAL CHILE, FAC CIENCIAS VETERINARIAS, 1996)
From a culture of Escherichia coli, strain B44, pili K99 was purified and used for generating murine monoclon al antibodies. These IgG1 antibodies, purified from ascitis by immunoaffinity Protein G-Sepharose chromatography, ...
Generation and evaluation of a monoclonal antibody anti-pili k99 from enteropathogenic escherichia coli
(UNIV AUSTRAL CHILE, FAC CIENCIAS VETERINARIAS, 1996)
From a culture of Escherichia coli, strain B44, pili K99 was purified and used for generating murine monoclon al antibodies. These IgG1 antibodies, purified from ascitis by immunoaffinity Protein G-Sepharose chromatography, ...
Improvement of enterocin P purification process
(Springer, 2006-09)
Purification and heterologous expression of enterocin P (EntP), a sec-dependent bacteriocin produced byEnterococcus faecium, inEscherichia coli is described. PCR-amplified product of the enterocin P structural geneentP was ...
Octadecyl silica: A solid phase for protein purification by immunoadsorption
(1991)
Immunoaffinity chromatography involves binding of an antigen or antibody to a solid matrix, usually agarose, frequently using the cyanogen bromide method. These methods are laborious, rather expensive, and their use has ...
A versatile ionic strength sensitive tag from a human GM CSF derived linear epitope
(Elsevier, 2013-06)
A 7-mer hGM-CSF-derived linear epitope (APARSPS) is herein described as a novel and small tag taking into account its particular binding affinity in native conditions that could be easily modified by increasing or lowering ...
Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form
(Springer, 2015)
Chagas disease, caused by the protozoan
Trypanosoma cruzi, is a major parasitic disease that affects
millions of people in America. However, despite the high
impact of this disease on human health, no effective and ...