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Biotechnological challenges: The scope of genome editing
(Sociedade Brasileira de Reproducao Assistida, 2021-02)
CRISPR/Cas9 can be considered as the biotechnological breakthrough of the century. Genome editing technologies have developed in a vertiginous way. While the genome editing of species, including animals, plants and bacteria ...
Towards a comprehensive picture of C-to-U RNA editing sites in angiosperm mitochondria
(Springer, 2018-06)
Key message: Our understanding of the dynamic and evolution of RNA editing in angiosperms is in part limited by the few editing sites identified to date. This study identified 10,217 editing sites from 17 diverse angiosperms. ...
Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors
(Wiley, 2022-07-14)
Viral vectors provide a quick and effective way to express exogenous sequences in eukaryotic cells and to engineer eukaryotic genomes through the delivery of CRISPR/Cas components. Here, we present JoinTRV, an improved ...
Comprehending the evolution of gene editing platforms for crop trait improvement
(Frontiers Media, 2022)
The CRISPR toolbox for the gram-positive model bacterium Bacillus subtilis
(2021-01-01)
CRISPR has revolutionized the way we engineer genomes. Its simplicity and modularity have enabled the development of a great number of tools to edit genomes and to control gene expression. This powerful technology was first ...
De novo domestication of wild tomato using genome editing
(Nature Biotechnology, 2019)
Developments and opportunities in fungal strain engineering for the production of novel enzymes and enzyme cocktails for plant biomass degradation
(2019-11-01)
Fungal strain engineering is commonly used in many areas of biotechnology, including the production of plant biomass degrading enzymes. Its aim varies from the production of specific enzymes to overall increased enzyme ...