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The use of Fluorescence Resonance Energy Transfer (FRET) peptidesfor measurement of clinically important proteolytic enzymes
(Academia Brasileira de Ciências, 2009-09-01)
Proteolytic enzymes have a fundamental role in many biological processes and are associated with multiple pathological conditions. Therefore, targeting these enzymes may be important for a better understanding of their ...
Characterization of angiotensin I-converting enzyme N-domain selectivity using positional-scanning combinatorial libraries of fluorescence resonance energy transfer peptides
(Walter de Gruyter & Co, 2012-12-01)
Somatic angiotensin I-converting enzyme (ACE) has two homologous active sites (N and C domains) that show differences in various biochemical properties. in a previous study, we described the use of positional-scanning ...
End-to-end distance distribution in bradykinin observed by Forster resonance energy transfer
(Elsevier B.V., 2000-04-06)
Forster resonance energy transfer (FRET) was used to study the conformational dynamics of bradykinin related peptides. the fluorescent probe aminobenzoic acid (Abz) bound to the amino terminal of bradykinin maintained its ...
Neprilysin carboxydipeptidase specificity studies and improvement in its detection with fluorescence energy transfer peptides
(Walter de Gruyter & Co, 2007-04-01)
We examined the substrate specificity of the carboxydipeptidase activity of neprilysin (NEP) using fluorescence resonance energy transfer (FRET) peptides containing ortho-aminobenzoyl (Abz) and 2,4-dinitrophenyl (Dnp) as ...
Analysis of catalytic properties of tripeptidyl peptidase I (TTP-I), a serine carboxyl lysosomal protease, and its detection in tissue extracts using selective FRET peptide substrate
(Elsevier Science Inc, 2016)
Tripeptidyl peptidase I (TPP-I), also named ceroid lipofuscinosis 2 protease (CLN2p), is a serine carboxyl lysosomal protease involved in neurodegenerative diseases, and has both tripeptidyl amino- and endopeptidase ...
A continuous fluorescence resonance energy transfer angiotensin I-converting enzyme assay
(Nature Publishing Group, 2006-01-01)
Angiotensin I-converting enzyme (ACE) is involved in various physiological and physiopathological conditions; therefore, the measurement of its catalytic activity may provide essential clinical information. This protocol ...
FRET peptides reveal differential proteolytic activation in intraerythrocytic stages of the malaria parasites Plasmodium berghei and Plasmodium yoelii
(ELSEVIER SCI LTD, 2011)
Malaria is still a major health problem in developing countries. It is caused by the protist parasite Plasmodium, in which proteases are activated during the cell cycle. Ca(2+) is a ubiquitous signalling ion that appears ...
Controlled peptide solvation in portion-mixing libraries of FRET peptides: Improved specificity determination for dengue 2 virus NS2B-NS3 protease and human cathepsin S
(Amer Chemical Soc, 2007-07-01)
The solubility of peptides in aqueous buffers used for the enzyme assays is a common limitation for all peptide libraries. in principle, the more water-soluble peptides are, the more susceptible they will be to peptidase ...
Biochemical and Functional Characterization of a Metalloprotease from the Thermophilic Fungus Thermoascus aurantiacus
(Amer Chemical Soc, 2009-10-14)
Protease production was carried out in solid state fermentation. The enzyme was purified through precipitation with ethanol at 72% followed by chromatographies in columns of Sephadex G75 and Sephacryl S100. It was purified ...
Purification and characterization of a new alkaline serine protease from the thermophilic fungus Myceliophthora sp.
(Elsevier B.V., 2011-11-01)
This work reports the purification of a novel alkaline protease enzyme from a putative new thermophilic fungus Myceliophthora sp. The molecular weight of the enzyme was determined as 28.2 kDa by using MALDI-TOF MS and it ...