Buscar
Mostrando ítems 1-10 de 4699
Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase
(Associação Brasileira de Divulgação Científica, 1998-10-01)
For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) ...
Nuclear and kinetoplast DNA synthesis in Trypanosoma cruzi, autoradiographical study with DNA polymerase inhibitors
(1993)
DNA synthesis in epimastigote forms of Trypanosoma cruzi was studied autoradiographically by incorporation of [3H]thymidine in the nuclear and kinetoplast DNA. Both DNA were heavily labelled. Three eukaryotic DNA polymerase ...
Purification and characterization of a β-like DNA polymerase from Trypanosoma cruzi
(1995)
A DNA polymerase was purified to near homogeneity from Trypanosoma cruzi epimastigotes. This preparation had a major polypeptide of 50 kDa and a minor band of 45 kDa. SDS-PAGE studies and a novel colorimetric activity gel ...
Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
(Nature Research, 2019)
INHIBITION OF DNA-POLYMERASE ALPHA AND REVERSE-TRANSCRIPTASE ACTIVITIES BY HPA-23
(Inst Tecnologia ParanaCuritiba-paranaBrasil, 1991)
Animal Mitochondrial DNA Replication
(2016-01-01)
Recent advances in the field of mitochondrial DNA (mtDNA) replication highlight the diversity of both the mechanisms utilized and the structural and functional organization of the proteins at mtDNA replication fork, despite ...
Remdesivir triphosphate blocks DNA synthesis and increases exonucleolysis by the replicative mitochondrial DNA polymerase, Pol γ
(2021-11-01)
The COVID-19 pandemic prompted the FDA to authorize a new nucleoside analogue, remdesivir, for emergency use in affected individuals. We examined the effects of its active metabolite, remdesivir triphosphate (RTP), on the ...
Inhibition of Taq DNA polymerase by catalpol.
(C M B Association, 2004-09)
DNA polymerases have recently emerged as important cellular targets for chemical intervention in the development of anti-cancer agents. This report describes a PCR assay as a method to investigate the action mechanism of ...
Molecular and functional characteristics of DNA polymerase beta-like enzymes from trypanosomatids
(Frontiers Media, 2021)
Trypanosomatids are a group of primitive unicellular eukaryotes that can cause diseases in plants, insects, animals, and humans. Kinetoplast genome integrity is key to trypanosomatid cell survival and viability. Kinetoplast ...