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Preparation of an affinity cryogel column for lysozyme purification
(2017-08-13)
Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite ...
Development of an affinity cryogel for one step purification of lysozyme from chicken egg white
(2017-02-15)
In this study, a supermacroporous polyacrylamide cryogel was produced by cryo-polymerization and activated with Tris(hydroxymethyl)aminomethane (Tris-cryogel) to be applied as an affinity ligand for a one step purification ...
Cryogels from albumin and low methoxyl amidated pectin as a matrix for betalain encapsulation
(2020-01-01)
Betalain from beetroot (Beta vulgaris) was encapsulated in cryogels consisting of albumin (ALB) and albumin-pectin (ALB-PEC). The color characteristics, encapsulation efficiency (EE), and release profile, as well as the ...
Production and capture of β-glucosidase from Thermoascus aurantiacus using a tailor made anionic cryogel
(2019-07-01)
In this study, an anion exchange cryogel was prepared and characterized using morphological, hydrodynamic and thermal techniques and it was used to capture β-glucosidase from Thermoascus aurantiacus. The enzyme was produced ...
High capacity cryogel-type adsorbents for protein purification
(Elsevier Science, 2014-06)
Cryogel bodies were modified to obtain epoxy groups by graft-copolymerization using both chemical and gamma irradiation initiation techniques. The free epoxy adsorbents were reacted further to introduce diethylaminoethanol ...
Hydrodynamics and dynamic capacity of cryogels produced with different monomer compositions
(Wiley-Blackwell, 2019-09-12)
Cryogels have received great attention in the area of bioseparation due to their characteristic of very large and interconnected pores. These materials allow rapid isolation of various biomolecules and permit that ...
Preparation and characterization of polyvinyl alcohol–pectin cryogels containing enrofloxacin and keratinase as potential transdermal delivery device
(VBRI Press AB, 2016-05-23)
Polyvinyl alcohol (PVA) and polyvinyl alcohol-pectin (PVA-P) cryogels are potential devices for wound healing. These materials have advantages over commercial wound dressings such as: retaining an appropriate level of ...
DEVELOPMENT OF A BIOREACTOR BASED ON LIPASE ENTRAPPED IN A MONOLITHIC CRYOGEL FOR ESTERIFICATION AND INTERESTERIFICATION REACTIONS
(Univ Autonoma Metropolitana-iztapalapa, 2018-04-01)
In this work, it was developed a novel bioreactor consisting of Aspergillus niger lipase (ANL) entrapped in a supermacroporous polyactylarnide cryogel. Bioreactor performance in catalyzing the flavor ester butyl butyrate ...