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Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems
(Mdpi, 2020-04-01)
Spermatogenesis is a continuous and dynamic developmental process, in which a single diploid spermatogonial stem cell (SSC) proliferates and differentiates to form a mature spermatozoon. Herein, we summarize the accumulated ...
Isolation, in vitro study, and stem cell markers for type A spermatogonia in a Characiformes species
(2020-07-01)
The objective of this study was to establish a protocol for the characterization, isolation, and culture of type A spermatogonia using specific molecular markers for these cells in fish. To this end, adult Prochilodus ...
Two short sequences from amaranth 11S globulin are sufficient to target green fluorescent protein and beta-glucuronidase to vacuoles in Arabidopsis cells
(Elsevier France-editions Scientifiques Medicales Elsevier, 2007-12)
Vacuolar sorting of seed storage proteins is a very complex process since several sorting pathways and interactions among proteins of different classes have been reported. In addition, although the C-terminus of several ...
Glycans in post-Golgi apical targeting: sorting signals or structural props?
(ELSEVIER SCIENCE LONDON, 1999)
A recent model proposed that N-glycans serve as apical targeting signals for soluble and membrane proteins in epithelial cells and neurons by interacting with lectin sorters in the trans-Golgi network. However, we believe ...
The rat liver Na+/bile acid cotransporter - Importance of the cytoplasmic tail to function and plasma membrane targeting
(AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2001)
To understand the potential functions of the cytoplasmic tail of Na+/taurocholate cotransporter (Ntcp) and to determine the basolateral sorting mechanisms for this transporter, green fluorescent protein-fused wild type and ...
AP1B sorts basolateral proteins in recycling and biosynthetic routes of MDCK cells
(NATL ACAD SCIENCES, 2007)
The epithelial-specific adaptor AP1B sorts basolateral proteins, but the trafficking routes where it performs its sorting role remain controversial. Here, we used an RNAi approach to knock down the medium subunit of AP1B ...
Magnetic-Activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations
(Humana Press, 2018)
This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, ...
Predecessor Search
(Assoc Computing Machinery, USA, 2020)
The predecessor problem is a key component of the fundamental sorting-and-searching core of algorithmic problems. While binary search is the optimal solution in the comparison model, more realistic machine models on integer ...