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A Multivariate Calibration Procedure for UV/VIS Spectrometric Monitoring of BHK-21 Cell Metabolism and Growth
(Wiley-Blackwell, 2014-01-01)
Monitoring mammalian cell culture with UV-vis spectroscopy has not been widely explored. The aim of this work was to calibrate Partial Least Squares (PLS) models from off-line UV-vis spectral data in order to predict some ...
Cell-to-cell transmission is the main mechanism supporting bovine viral diarrhea virus spread in cell culture
(American Society for Microbiology, 2019-01)
After initiation of an infective cycle, spread of virus infection can occur fundamentally in two different ways: (i) viral particles can be released into the external environment and diffuse through the extracellular space ...
The role of mitochondria and biotransformation in abamectin-induced cytotoxicity in isolated rat hepatocytes
(2013-03-01)
Abamectin (ABA), which belongs to the family of avermectins, is used as a parasiticide; however, ABA poisoning can impair liver function. In a previous study using isolated rat liver mitochondria, we observed that ABA ...
The role of mitochondria and biotransformation in abamectin-induced cytotoxicity in isolated rat hepatocytes
(2013-03-01)
Abamectin (ABA), which belongs to the family of avermectins, is used as a parasiticide; however, ABA poisoning can impair liver function. In a previous study using isolated rat liver mitochondria, we observed that ABA ...
Oxidative stress monitoring in in vitro and in vivo models
(2020-01-01)
In aerobic organisms, cellular respiration is an essential process that is divided into several steps. The third and last step is called oxidative phosphorylation, which occurs in the mitochondria, specifically in the inner ...
Isolation, in vitro study, and stem cell markers for type A spermatogonia in a Characiformes species
(2020-07-01)
The objective of this study was to establish a protocol for the characterization, isolation, and culture of type A spermatogonia using specific molecular markers for these cells in fish. To this end, adult Prochilodus ...
Hybrid-EKF: Hybrid model coupled with extended Kalman filter for real-time monitoring and control of mammalian cell culture
(John Wiley & Sons Inc, 2020-05)
In a decade when Industry 4.0 and quality by design are major technology drivers of biopharma, automated and adaptive process monitoring and control are inevitable requirements and model-based solutions are key enablers ...