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Microneedles rollers as a potential device to increase ALA diffusion and PpIX production: Evaluations by wide-field fluorescence imaging and fluorescence spectroscopy
(2014-01-01)
One of the limitations of topical photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) is the poor ability to penetrate biological barriers of skin and the recurrence rates in treatments. This study aimed to identify ...
Microneedles rollers as a potential device to increase ALA diffusion and PpIX production: evaluations by wide-field fluorescence imaging and fluorescence spectroscopy
(International Society for Optical Engineering - SPIEBellingham, 2014)
One of the limitations of topical photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) is the poor ability to penetrate biological barriers of skin and the recurrence rates in treatments. This study aimed to identify ...
Single Molecule Blinking and Photobleaching Separated by Wide-Field Fluorescence Microscopy
(American Chemical Society, 2005-08)
Single molecule fluorescence detection of Atto590 in poly(vinyl alcohol) was achieved by using a wide-field epifluorescence microscope with CCD-camera detection. Image sequences are obtained from which the time traces of ...
Mapping pixel dissimilarity in wide-field super-resolution fluorescence microscopy
(American Chemical Society, 2015-05)
Recent advances in fluorescence bioimaging with single-molecule sensitivity have relied on the analysis and visualization of single-molecule data obtained on smart fluorophores. We describe an alternative method to enhance ...
Superresolution method for a single wide-field image deconvolution by superposition of point sources
(Wiley Blackwell Publishing, Inc, 2019-05-07)
In this work, we present a new algorithm for wide‐field fluorescent micrsocopy deconvolution from a single acquisition without a sparsity prior, which allows the retrieval of the target function with superresolution, with ...
Monitoring scaling and dental calculus removal with an optical fluorescence system
(Institute of Physics - IOPBristol, 2014-08)
Fluorescence results from a process that occurs under certain conditions in molecules known as fluorophores, fluorochromes or fluorescent dyes when they absorb light. The molecule is excited to a higher energy state and ...
Monitoring scaling and dental calculus removal with an optical fluorescence system
(Iop Publishing Ltd, 2014-08-01)
Fluorescence results from a process that occurs under certain conditions in molecules known as fluorophores, fluorochromes or fluorescent dyes when they absorb light. The molecule is excited to a higher energy state and ...
Identification of skin lesions through aminolaevulinic acid-mediated photodynamic detection
(Elsevier BVAmsterdam, 2014-09)
Non-melanoma skin cancer is the most common cancer lesion worldwide. In Brazil, it represents 95% of all skin cancer lesions, and 25% of all tumor types. Early diagnosis allows treatment at initial stages of the disease, ...
Single image deconvolution with super-resolution using the SUPPOSe algorithm
(SPIE, 2019-02)
We present the results of super-resolution deconvolution of fluorescent intracellular images using the SUPPOSe algorithm. The image is acquired using a standard fluorescence microscope and a CMOs low noise high dynamic ...
Comparison of multispectral wide-field optical imaging modalities to maximize image contrast for objective discrimination of oral neoplasia
(SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS, 2010)
Multispectral widefield optical imaging has the potential to improve early detection of oral cancer. The appropriate selection of illumination and collection conditions is required to maximize diagnostic ability. The goals ...