We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. ...

The principal motor tract involved in mammalian locomotor activities is known as the corticospinal tract (CST), which starts in the brain motor cortex (upper motor neuron), extends its axons across the brain to brainstem ...

Multiphoton microscopy is a powerful technique for imaging due to its deep penetration, low scattering and sectioning power, allowing control on all three axes for both imaging and molecular actuation, but involves expensive ...

The aim of this chapter is to introduce an experimental approach that allows visualization of temperature-dependent lipid phase equilibria at the level of single vesicles, using two-photon fluorescence microscopy. Various ...

This chapter describes giant vesicles, Laurdan, and two-photon fluorescence microscopy. The combination of Laurdan, giant unilamellar vesicles (GUVs), and two-photon fluorescence microscopy has been extremely useful in ...