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Basic protocols for Drosophila S2 cell line: maintenance and transfection
(Humana Press, 2007-12)
Cells in culture have been increasingly employed in the dissection of intracellular processes. They are generally easier to handle than the organism of study and certainly less complex, which facilitates testing for specific ...
Optimization of polyethylenimine-mediated transient transfection using response surface methodology design
(Pontificia Universidad Católica de Valparaíso, 2010)
Avaliação da expressão transiente do gene da glicoproteína do vírus da raiva (RVGP) em células de inseto da linhagem Drosophila melanogaster S2
(Universidade Federal de São CarlosBRUFSCarPrograma de Pós-Graduação em Biotecnologia - PPGBiotec, 2011-11-22)
Rabies is a zoonotic viral disease caused by a virus of the genus Lyssavirus that affects several species of mammals. Rabies remains a global public health threat that kills more than 55,000 people per year mainly in ...
Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII
(London, 2011)
Abstract
Background
Hemophilia A is a bleeding disorder caused by deficiency in coagulation factor VIII. Recombinant factor ...
Effective gene delivery to Trypanosoma cruzi epimastigotes through nucleofection
(Japanese Society of Parasitology, 2017-01)
New opportunities have raised to study the gene function approaches of Trypanosoma cruzi after its genome sequencing
in 2005. Functional genomic approaches in Trypanosoma cruzi are challenging due to the reduced ...
46 meganuclease i-SceI enhances stable transgene integration in cultured bovine fetal fibroblasts
(Csiro Publishing, 2012-12)
Production of genetically modified large animals through somatic cell nuclear transfer (SCNT) requires genetic manipulation of cultured cells, which are subsequently used as nuclear donors to generate a transgenic animal. ...