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Computer match prediction for fluorescent dyes by neural networks
(John Wiley & Sons, 2006-06-22)
Fluorescent dyes present difficulties for match prediction due to their variable excitation and emission characteristics, which depend on a variety of factors. An empirical approach is therefore favoured, such as that used ...
From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments
(American Physical Society, 2013-02-13)
Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with ...
Single Molecule Blinking and Photobleaching Separated by Wide-Field Fluorescence Microscopy
(American Chemical Society, 2005-08)
Single molecule fluorescence detection of Atto590 in poly(vinyl alcohol) was achieved by using a wide-field epifluorescence microscope with CCD-camera detection. Image sequences are obtained from which the time traces of ...
Combining modules for versatile and optimal labeling of lactic acid bacteria: Two pMV158-family promiscuous replicons, a pneumococcal system for constitutive or inducible gene expression, and two fluorescent proteins
(Frontiers Media S.A., 2019-06)
Labeling of bacterial cells with fluorescent proteins allows tracking the bacteria in competition and interactomic in vivo and in vitro studies. During the last years, a few plasmid vectors have been developed aimed at the ...
Quantification of fluctuations from fluorescence correlation spectroscopy experiments in reaction-diffusion systems
(American Physical Society, 2020-11)
Fluorescence correlation spectroscopy (FCS) is commonly used to estimate diffusion and reaction rates. In FCS the fluorescence coming from a small volume is recorded and the autocorrelation function (ACF) of the fluorescence ...
Mapping the lignin distribution in pretreated sugarcane bagasse by confocal and fluorescence lifetime imaging microscopy
(BioMed CentralLondon, 2013-04)
Background: Delignification pretreatments of biomass and methods to assess their efficacy are crucial for biomassto- biofuels research and technology. Here, we applied confocal and fluorescence lifetime imaging microscopy ...
Spectroscopic characterization of 2-amino-N-hexadecyl-benzamide (AHBA), a new fluorescence probe for membranes
(Elsevier B.V., 2006-11-20)
We report the results of investigation on the spectroscopic properties of a new fluorescent lipophylic probe. the fluorophore o-aminobenzoic acid was covalently bound to the acyl chain hexadecylamine, producing the compound ...
Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?
(Elsevier Science Bv, 2018)
In this work, fluorescence lifetime has been used to analyze protoporphyrin IX (PPIX) extracted from blood for diagnosing atherosclerosis. A total of 10 adult white male rabbits (New Zealand) were divided into the control ...