Buscar
Mostrando ítems 1-10 de 1367
Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds
(2018-07-01)
BACKGROUND: Green fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) ...
Application of response surface methodology and artificial neural networks for optimization of recombinant Oryza sativa non-symbiotic hemoglobin 1 production by escherichia coli in medium containing byproduct glycerol
(Elsevier, 2010-10)
Production of recombinant Oryza sativa non-symbiotic hemoglobin 1 (OsHb1) by Escherichia coli was maximized in shake-flask cultures in media containing tryptone, yeast extract, sodium chloride and byproduct glycerol from ...
Effect of recombinant boar beta-acrosin on sperm binding to intact zona pellucida during in vitro fertilization
(1999)
In a previous paper we demonstrated that boar β-acrosin recombinant proteins were able to bind non-enzymatically to solubilized pig zona pellucida (ZP) glycoproteins. Here we report the participation of boar β-acrosin in ...
A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins
(ELSEVIER SCI LTD, 2006)
We report here the protective effect against piscirickettsiosis elicited in fish by a mixture of recombinant proteins. A comparative genomics strategy was used on a genomic library of Piscirickettsia salmonis in order to ...
Origins of sequence diversity in the malaria vaccine candidate merozoite surface protein-2 (MSP-2) in Amazonian isolates of Plasmodium falciparum
(Elsevier B.V., 2006-07-19)
The recent evolution of Plasmodium falciparum is at odds with the extensive polymorphism found in most genes coding for antigens. Here, we examined the patterns and putative mechanisms of sequence diversification in the ...
Origins of sequence diversity in the malaria vaccine candidate merozoite surface protein-2 (MSP-2) in Amazonian isolates of Plasmodium falciparum
(Elsevier B.V., 2006-07-19)
The recent evolution of Plasmodium falciparum is at odds with the extensive polymorphism found in most genes coding for antigens. Here, we examined the patterns and putative mechanisms of sequence diversification in the ...
Optimal Conditions for Biomass and Recombinant Glycerol Kinase Production Using the Yeast Pichia pastoris
(Faculty Food Technology Biotechnology, 2011-07-01)
The extracellular glycerol kinase gene from Saccharomyces cerevisiae (GUT]) was cloned into the expression vector pPICZ alpha. A and integrated into the genome of the methylotrophic yeast Pichia pastoris X-33. The presence ...
Optimal conditions for biomass and recombinant glycerol kinase production using the yeast Pichia pastoris
(2011-07-01)
The extracellular glycerol kinase gene from Saccharomyces cerevisiae (GUT1) was cloned into the expression vector pPICZα A and integrated into the genome of the methylotrophic yeast Pichia pastoris X-33. The presence of ...