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Estudo de metodologia para avaliação de estreptoquinase em produtos biofarmacêuticos
(Universidade Federal de Santa MariaBRFarmacologiaUFSMPrograma de Pós-Graduação em Ciência e Tecnologia Farmacêuticas, 2013-07-26)
Streptokinase is clinically used as a thrombolytic agent for the treatment of patients with
acute myocardial infarction and venous and arterial thrombosis. In the present work, a substrate
chromogenic assay was validated ...
Improved expression of green fluorescent protein in cattle embryos produced by ICSI-mediated gene transfer with spermatozoa treated with streptolysin-O
(Elsevier Science, 2018-09)
The ICSI-sperm mediated gene transfer (ICSI-SMGT) has been used to produce transgenic mice with high efficiency; however, the efficiency of this technique in farm animals is still less than desirable. Pretreatment of sperm ...
Membrane-permeable Rab27A is a regulator of the acrosome reaction: Role of geranylgeranylation and guanine nucleotides
(Elsevier Science Inc, 2018-04)
The acrosome reaction is the regulated exocytosis of mammalian sperm's single secretory granule, essential for fertilization. It relies on small GTPases, the cAMP binding protein Epac, and the SNARE complex, among other ...
Erythema elevatum diutinum presenting with a giant annular pattern
(WILEY-BLACKWELL PUBLISHING, INC, 2009)
A 45-year-old woman presented with a 3-year history of cutaneous lesions. Clinical examination revealed red-yellow to brownish infiltrated papules coalescing into annular-shaped plaques of several sizes with a hyperpigmented ...
The rab3A-22A chimera prevents sperm exocytosis by stabilizing open fusion pores
(American Society for Biochemistry and Molecular Biology, 2016-10)
At the final stage of exocytotis, a fusion pore opens between the plasma and a secretory vesicle membranes; typically, when the pore dilates the vesicle releases its cargo. Sperm contain a large dense-core secretory granule ...
Cardiomyogenic differentiation of bone marrow mesenchymal cells: role of cardiac extract from neonatal rat cardiomyocytes
(Elsevier, 2010-02)
Bone marrow mesenchymal stromal cells (BM-MSCs) with regenerative potential have been identified in heart. Whether these cells become new cardiac lineage cells by phenomena of transdifferentiation or fusion is also being ...
Epac, Rap and Rab3 act in concert to mobilize calcium from sperm's acrosome during exocytosis
(BioMed Central, 2014-08)
BACKGROUND: Exocytosis of sperm's single secretory granule or acrosome (acrosome reaction, AR) is a highly regulated event essential for fertilization. The AR begins with an influx of calcium from the extracellular milieu ...
Prevalence of Microorganisms and Immunoglobulins in Children with Tonsillar Hypertrophy and Adenoiditis
(Fundação Otorrinolaringologia, 2014-09-01)
Introduction: Benign idiopathic tonsillar hypertrophy (HBI) may affect a child's quality of life and sleep. Several studies have sought to relate the clinical features of HBI with the infectious and/or immunologic changes ...
GTP-bound Rab3A exhibits consecutive positive and negative roles during human sperm dense-core granule exocytosis
(Oxford University Press, 2014-08)
Exocytosis of mammalian sperm dense-core secretory granule relies on the same fusion molecules as all other secretory cells; one such molecule is the small GTPase Rab3A. Here, we report an in-depth biochemical characterization ...