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Comparative steady-state fluorescence studies of cytosolic rat liver (GTP), Saccharomyces cerevisiae (ATP) and Escherichia coli (ATP) phosphoenolpyruvate carboxykinases
(1993)
Two members of the ATP-dependent class of phosphoenolpyruvate (PEP) carboxykinases (Saccharomyces cerevisiae and Escherichia coli PEP carboxykinase), and one member of the GTP-dependent class (the cytosolic rat liver enzyme) ...
β-sheet to α-helix conversion and thermal stability of β-Galactosidase encapsulated in a nanoporous silica gel
(Academic Press Inc Elsevier Science, 2019-01-01)
The effect on protein conformation and thermal stability was studied for β-Galactosidase (β-Gal) encapsulated in the nanopores of a silicate matrix (Eβ-Gal). Circular dichroism spectra showed that, compared with the enzyme ...
β-Galactosidase at the membrane–water interface: a case of an active enzyme with non-native conformation
(Elsevier Science, 2013-02)
Previously we demonstrated that Escherichia coli beta-galactosidase (β-Gal) binds to zwitterionic lipid membranes improving its catalytic activity. To understand the activation mechanism from the protein perspective, here ...
Laurdan Studies of Membrane Lipid-Acetylcholine Receptor Protein Interactions
(Humana Press, 2007)
The extrinsic fluorescent probe Laurdan (6-dodecanoyl-2-dimethylamino naphthalene) exhibits extreme sensitivity to the polarity and to the molecular dynamics of the dipoles in its environment. Dipolar relaxation processes ...
Tubulin equilibrium unfolding followed by time-resolved fluorescence and fluorescence correlation spectroscopy
(2004)
The pathway for the in vitro equilibrium unfolding of the tubulin heterodimer by guanidinium chloride (GdmCl) has been studied using several spectroscopic techniques, specifically circular dichroism (CD), two-photon ...
Insights into the HyPer biosensor as molecular tool for monitoring cellular antioxidant capacity
(Elsevier B.V., 2018)
© 2018 The Authors Aerobic metabolism brings inexorably the production of reactive oxygen species (ROS), which are counterbalanced by intrinsic antioxidant defenses avoiding deleterious intracellular effects. Redox balance ...
FAD binding properties of a cytosolic version of Escherichia coli NADH dehydrogenase-2
(Elsevier Science, 2014-03)
Respiratory NADH dehydrogenase-2 (NDH-2) of Escherichia coli is a peripheral membrane-bound flavoprotein. By eliminating its C-terminal region, a water soluble truncated version was obtained in our laboratory. Overall ...
Enhancement of Photophysical and Photosensitizing properties of Flavin Adenine Dinucleotide by Mutagenesis of the C-terminal extension of a bacterial Flavodoxin Reductase
(Wiley Vch Verlag, 2015-03)
The role of the mobile C-terminal extension present in Rhodobacter capsulatus ferredoxin–NADP(H) reductase (RcFPR) was evaluated using steady-state and dynamic spectroscopies for both intrinsic Trp and FAD in a series of ...
Effect of organochlorine insecticides on nicotinic acetylcholine receptor-rich membranes
(Pergamon-Elsevier Science Ltd, 2000-05)
The so-called generalized polarization (GP) of the fluorescent probe Laurdan and the steady-state fluorescence anisotropy of the probe diphenylhexatriene (DPH) and its phenylpropionic derivative (PA-DPH) were used to study ...
Mecanismo y evolución de la activación por AMP en quinasas de azúcares dependientes de ADP de Archaea
(Universidad de Chile, 2020-05)
Las enzimas que fosforilan glucosa y fructosa-6-fosfato (F6P) en la glicólisis de la mayoría de los organismos del phylum Euryarchaeota de Archaea utilizan ADP en lugar de ATP como dador de fosforilo. Estas enzimas están ...