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Evaluation of microbiological variants of sputum processing and concentration of mycobacteria to optimize the microscopic and imaging diagnosis of tuberculosis
(Medknow Publications, 2019)
Background: Direct sputum smear is still the first-choice tool for screening of tuberculosis worldwide. Variants of this technique, to improve the sensitivity are desired. Methods: Two microbiological variants of the ...
Induced sputum is safe and well-tolerated for TB diagnosis in a resource-poor primary healthcare setting
(American Society of Tropical Medicine and Hygiene, 2015)
Improved tuberculosis (TB) diagnostics are required. Induced sputum sampling is superior to spontaneous sputum analysis for diagnosis of pulmonary TB. Therefore, we examined the applicability of induced sputum in primary ...
Evaluation of bleach-sedimentation for sterilising and concentrating Mycobacterium tuberculosis in sputum specimens
(BioMed Central, 2011)
Background: Bleach-sedimentation may improve microscopy for diagnosing tuberculosis by sterilising sputum and concentrating Mycobacterium tuberculosis. We studied gravity bleach-sedimentation effects on safety, sensitivity, ...
Sputum cytology in the diagnosis of pulmonary paracoccidioidomycosis
(1991-06-01)
The presence of Paracoccidioides brasiliensis was determined in sputum samples from 50 patients with paracoccidioidomycosis using four different techniques: (a) cell-block preparations stained with silver methenamine, (b) ...
Sputum cytology in the diagnosis of pulmonary paracoccidioidomycosis
(1991-06-01)
The presence of Paracoccidioides brasiliensis was determined in sputum samples from 50 patients with paracoccidioidomycosis using four different techniques: (a) cell-block preparations stained with silver methenamine, (b) ...
A novel algorithm for detection of tuberculosis bacilli in sputum smear fluorescence images
(Institute of Advanced Engineering and Science, 2020)
This work proposes an algorithm aimed at recognizing and accounting Koch bacilli in digital images of microbiological sputum samples stained with auramine, in order to determine the degree of concentration and the state ...
Standardization of a TaqMan-based real-time PCR for the detection of Mycobacterium tuberculosis-complex in human sputum
(American Society of Tropical Medicine and Hygiene, 2014)
Real-time polymerase chain reaction (qPCR) was optimized for detecting Mycobacterium tuberculosis in sputum. Sputum was collected from patients (N = 112) with suspected pulmonary tuberculosis, tested by smear microscopy, ...
Evaluation of Microscopic Observation Drug Susceptibility (MODS) and the string test for rapid diagnosis of pulmonary tuberculosis in HIV/AIDS patients in Bolivia
(BioMed Central, 2015)
Background: Tuberculosis (TB) is the most common opportunistic infection and the leading cause of death in HIV-positive people worldwide. Diagnosing TB is difficult, and is more challenging in resource-scarce settings where ...
Comparison of sputum collection methods for tuberculosis diagnosis: a systematic review and pairwise and network meta-analysis
(Elsevier, 2017)
BACKGROUND: The performance of laboratory tests to diagnose pulmonary tuberculosis is dependent on the quality of the sputum sample tested. The relative merits of sputum collection methods to improve tuberculosis diagnosis ...