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Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis
(2018-11-01)
The identification of distinct proteins present on the membrane of spermatozoa with X and Y chromosomes allows the development of immuno-sexing techniques. The aim of this study, therefore, was to use mass spectrometry to ...
Effect of different mini-volume colloid centrifugation configurations on flow cytometrically sorted sperm recovery efficiency and quality using a computer-assisted semen analyzer
(Wiley Blackwell Publishing, Inc, 2018-02)
Straws of sex-sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 106 sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of ...
Sperm sexing mediated by magnetic nanoparticles in donkeys, a preliminary in vitro study
(Elsevier Science Inc, 2018-06)
Preselection of sex before conception has been one of the objectives pursued by scientists and breeders for many years. The donkey milk industry has shown a greater commercial interest, since jennies’ demand is increasing ...
Sperm pretreatment with heparin and L-glutathione, sex-sorting, and double cryopreservation to improve intracytoplasmic sperm injection in bovine
(Elsevier Science Inc, 2017-04)
In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with ...
Flow cytometry sex sorting affects bull sperm longevity and compromises their capacity to bind to oviductal cells
(Elsevier B.V., 2018-01-01)
This study assessed the effect of flow cytometry sexing on sperm longevity and the capacity of sperm to bind to oviductal cells. Each ejaculate from four bulls was divided into two fractions: the first was immediately ...
Effect of different mini‐volume colloid centrifugation configurations on flow cytometrically sorted sperm recovery efficiency and quality using a computer‐assisted semen analyzer
(Wiley, 2018-02)
Straws of sex‐sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 106 sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of ...
Effects of sex-sorted spermatozoa on the efficiency of in vitro fertilization and ultrastructure of in vitro produced bovine blastocysts
(Blackwell Publishing, 2008-02)
Frozen-thawed sexed semen from six bulls (Holstein) was used for studying their efficiency in an in vitro fertilization (IVF)-programme and to compare their ultrastructure with in vitro produced bovine blastocysts produced ...
Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves
(2019-09-01)
Due to the increasing use of in vitro embryo production (IVEP) and the importance of crossbreeding for beef production, pregnancy rates of Nelore recipients were evaluated following Fixed Time Embryo Transfer with fresh ...