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Detection of Mycoplasma sp. and Acholeplasma laidlawii in biological products and culture media by real time PCR
(Fiocruz/Instituto de Tecnologia em Imunobiológicos, 2018)
Optimization of the Sybr Green real time PCR for the detection of Human Herpes Virus type 6 (HHV-6)
(Instituto de Medicina Tropical, 2008)
HHV-6 is the etiological agent of Exanthem subitum which is considered the sixth most frequent disease in infancy. In immuno-compromised hosts, reactivation of latent HHV-6 infection may cause severe acute disease. We ...
GP5+/6+ SYBR Green methodology for simultaneous screening and quantification of human papillomavirus
(ELSEVIER SCIENCE BV, 2009)
Background: Detection and quantification of human papillomavirus (HPV) may help in predicting the evolution of HPV infection and progression of associated lesions. Objectives: We propose a novel protocol using consensus ...
Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos
(2013-10-01)
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...
Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos
(2013-10-01)
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...
Development and validation of two SYBR green PCR assays and a multiplex real-time PCR for the detection of Shiga toxin-producing Escherichia coli in meat
(Elsevier Science, 2015-12)
Shiga toxin-producing Escherichia coli (STEC) are recognized as food-borne pathogens. We developed and validated two SYBR green PCR (SYBR-PCR) and a real-time multiplex PCR (RT-PCR) to detect stx1 and stx2 genes in meat ...
Detecção por PCR em tempo real e identificação molecular do Banana streak virus e Cucumber mosaic virus de Musa sppReal Time PCR detection and molecular identification of Banana streak virus and Cucucumber mosaic virus from Musa spp
(Universidade Estadual de MaringáBrasilDepartamento de AgronomiaPrograma de Pós-Graduação em AgronomiaUEMMaringá, PRCentro de Ciências Agrárias, 2018)