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Flow cytometric analysis using SYBR Green I for genome size estimation in coffee
(Acta Histochemica, 2018)
GP5+/6+ SYBR Green methodology for simultaneous screening and quantification of human papillomavirus
(ELSEVIER SCIENCE BV, 2009)
Background: Detection and quantification of human papillomavirus (HPV) may help in predicting the evolution of HPV infection and progression of associated lesions. Objectives: We propose a novel protocol using consensus ...
Ethidium bromide and SYBR Green I interact with lipid micelles
(OMICS International, 2017-03)
Ethidium Bromide and SYBR Green I are usual examples of ligands that specifically binds to DNA by intercalation. Here we describe that micelles of gangliosides, a lipidic family of sialoglycosphingolipids which are the ...
Detection of Mycoplasma sp. and Acholeplasma laidlawii in biological products and culture media by real time PCR
(Fiocruz/Instituto de Tecnologia em Imunobiológicos, 2018)
Optimization of the Sybr Green real time PCR for the detection of Human Herpes Virus type 6 (HHV-6)
(Instituto de Medicina Tropical, 2008)
HHV-6 is the etiological agent of Exanthem subitum which is considered the sixth most frequent disease in infancy. In immuno-compromised hosts, reactivation of latent HHV-6 infection may cause severe acute disease. We ...
Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos
(2013-10-01)
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...
Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos
(2013-10-01)
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...