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ProtMiscuity: a database of promiscuous proteins
(Oxford University Press, 2019-01)
Promiscuous behaviour in proteins and enzymes remains a challenging feature to understand the structure-function relationship. Here we present ProtMiscuity, a manually curated online database of proteins showing catalytic ...
Allosteric control of substrate specificity of the Escherichia coli ADP-glucose pyrophosphorylase
(Frontiers Research Foundation, 2017-06)
The substrate specificity of enzymes is crucial to control the fate of metabolites to different pathways. However, there is growing evidence that many enzymes can catalyze alternative reactions. This promiscuous behavior ...
Allosteric Control of Substrate Specificity of the Escherichia coli ADP-Glucose Pyrophosphorylase
(Frontiers Research Foundation, 2017-06)
The substrate specificity of enzymes is crucial to control the fate of metabolites to different pathways. However, there is growing evidence that many enzymes can catalyze alternative reactions. This promiscuous behavior ...
Hyperstability and substrate promiscuity in laboratory resurrections of precambrian β-lactamases
(American Chemical Society, 2013-02)
We report a sequence reconstruction analysis targeting several Precambrian nodes in the evolution of class-A β-lactamases and the preparation and experimental characterization of their encoded proteins. Despite extensive ...
Emergence of pyridoxal phosphorylation through a promiscuous ancestor during the evolution of hydroxymethyl pyrimidine kinases
(Elsevier, 2014)
In the family of ATP-dependent vitamin kinases, several bifunctional enzymes that phosphorylate
hydroxymethyl pyrimidine (HMP) and pyridoxal (PL) have been described besides enzymes specific
towards HMP. To determine how ...
Promiscuous subunit interactions: A possible mechanism for the regulation of protein kinase CK2
(1998)
Protein kinase CK2 is a ubiquitous eukaryotic ser/thr protein kinase. The active holoenzyme is a heterotetrameric protein corn posed of catalytic (α and α') and regulatory (β) subunits that phosphorylates many different ...
The UDP-glucose pyrophosphorylase from Giardia lamblia is redox regulated and exhibits promiscuity to use galactose-1-phosphate
(Elsevier Science, 2015-01)
Background: Giardia lamblia is a pathogen of humans and other vertebrates. The synthesis of glycogen and of structural oligo and polysaccharides critically determine the parasite's capacity for survival and pathogenicity. ...
Bioinspired 'Fe POT. III''Cd POT. II' and 'Fe POT. III''Hg POT. II' complexes: synthesis, characterization and promiscuous catalytic activity evaluation
(ElsevierPhiladelphia, 2011-12)
In this work we report on the synthesis, crystal structure, and physicochemical characterization of the novel dinuclear [FeIIICdII(L)(μ-OAc)2]ClO4·0.5H2O (1) complex containing the unsymmetrical ligand H2L = 2-bis[{(2-py ...
Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases
(American Society for Biochemistry and Molecular Biology, 2017)
One central goal in molecular evolution is to pinpoint the mechanisms and evolutionary forces that cause an enzyme to change its substrate specificity; however, these processes remain largely unexplored. Using the glycolytic ...
Molecular Basis Of Substrate Recognition And Specificity Revealed In Family 12 Glycoside Hydrolases
(Wiley-BlackwellHoboken, 2016)