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Basic protocols for Drosophila S2 cell line: maintenance and transfection
(Humana Press, 2007-12)
Cells in culture have been increasingly employed in the dissection of intracellular processes. They are generally easier to handle than the organism of study and certainly less complex, which facilitates testing for specific ...
Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occ- baculovirus occlusion and larval per os infectivity
(Elsevier Science, 2010-01)
Oral infection of insect larvae with baculovirus is an advantageous methodology for producing high levels of recombinant proteins and for achieving plague control. However, many recombinant baculoviruses express a foreign ...
Characterization of hepatitis B virus surface antigen particles expressed in stably transformed mammalian cell lines containing the large, middle and small surface protein
(Elsevier Science, 2020-11)
Vaccination still represents the most efficient and inexpensive strategy in the control of hepatitis B virus (HBV) infection. However, about 10% of the population vaccinated with the current yeast derived vaccine, consisting ...
Comparison of transient and stable expression of foot-and-mouth disease virus capsid proteins in mammalian cells
(Scientific Research Publishing, 2013-09)
Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant ...
Stable and high-level production of recombinant Factor IX in human hepatic cell line
(WILEY-BLACKWELL, 2011)
Hemophilia B is a genetic disease of the coagulation system that affects one in 30,000 males worldwide. Recombinant human Factor IX (rhFIX) has been used for hemophilia B treatment, but the amount of active protein generated ...
Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells
(BioMed Central Ltd., 2019)
Background: The production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins ...
Comparison of transient and stable expression of foot-and-mouth disease virus capsid proteins in mammalian cells
(Scientific Research, 2013-12)
Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant ...
Establishment of a Brazilian Line of Human Embryonic Stem Cells in Defined Medium: Implications for Cell Therapy in an Ethnically Diverse Population
(COGNIZANT COMMUNICATION CORP, 2011)
Pluripotent human embryonic stem (hES) cells are an important experimental tool for basic and applied research, and a potential source of different tissues for transplantation. However, one important challenge for the ...