Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) ...

The use of long ssDNA polynucleotides in PCR of degraded DNA samples can discriminate Short Tandem Repeat (STR) genotypes otherwise missed by current commercial kits.

On degraded DNA samples a multiplex PCR mixture containing polynucleotide primers can provide superior genotyping of Short Tandem Repeats (STRs) to the current commercial kits based on standard oligonucleotides.