Buscar
Mostrando ítems 1-10 de 450
Solid-phase peptide synthesis in highly loaded conditions
(Academic Press Inc. Elsevier B.V., 2011-02-01)
The use of very highly substituted resins has been avoided for peptide synthesis due to the aggravation of chain-chain interactions within beads. To better evaluate this problem, a combined solvation-peptide synthesis ...
Solid-phase peptide synthesis in highly loaded conditions
(Academic Press Inc. Elsevier B.V., 2011-02-01)
The use of very highly substituted resins has been avoided for peptide synthesis due to the aggravation of chain-chain interactions within beads. To better evaluate this problem, a combined solvation-peptide synthesis ...
Ca(2+)-mediated thiolysis of peptide-resin linkage as an option for preparing protected peptide thioesters
(PERGAMON-ELSEVIER SCIENCE LTD, 2008)
A mild new procedure for preparing protected peptide thioesters, based oil Ca(2+)-assisted thiolysis of peptide-Kaiser oxime resin (KOR) linkage, is described. Ac-Ile-Ser(Bzl)-Asp(OcHx)-SR (Ac: acetyl; Bzl: benzyl; cHx: ...
Comparative investigation of the cleavage step in the synthesis of model peptide resins: Implications for N-alpha-9-fluorenylmethyloxycarbonyl-solid phase peptide synthesis
(Pharmaceutical Soc Japan, 2007-03-01)
Based on our studies of the stability of model peptide-resin linkage in acid media, we previously proposed a rule for resin selection and a final cleavage protocol applicable to the N-alpha-tert-butyloxycarbonyl (Boc)-peptide ...
Study of the effect of the peptide loading and solvent system in SPPS by HRMAS-NMR
(Wiley-Blackwell, 2005-09-01)
The SPPS methodology has continuously been investigated as a valuable model to monitor the solvation properties of polymeric materials. In this connection, the present work applied HRMAS-NMR spectroscopy to examine the ...
Study of the effect of the peptide loading and solvent system in SPPS by HRMAS-NMR
(Wiley-Blackwell, 2005-09-01)
The SPPS methodology has continuously been investigated as a valuable model to monitor the solvation properties of polymeric materials. In this connection, the present work applied HRMAS-NMR spectroscopy to examine the ...
Design of affinity chromatography peptide ligands through combinatorial peptide libraries screening
(Springer, 2020)
In this chapter, a protocol to design affinity chromatography matrices with short peptide ligands immobilized for protein purification is described. The first step consists of the synthesis of a combinatorial peptide library ...
Peptide synthesis: chemical or enzymatic
(Pontificia Universidad Católica de Valparaíso, 2007)
Regioselective Incorporation of Backbone Constraints Compatible with Traditional Solid-Phase Peptide Synthesis
(American Chemical Society, 2012-12)
A protected aldehyde was attached via a twocarbon spacer to a peptide backbone amide nitrogen during a traditional Merrifield solid-phase synthesis. Acid-mediated unmasking of the aldehyde triggered the regioselective ...