The development of a sandwich-type biosensor for glucose quantification is presented. This work is focused on the optimization of the enzymatic matrix of the biosensor. The best performance was found for an enzymatic matrix ...

Most sandwich-type biosensors can be used several times, since their long-time stability generally ranges from weeks to several months. However, the response-time of this kind of sensors is typically slow because reagents ...

Most sandwich-type biosensors can be used several times, since their long-time stability generally ranges from weeks to several months. However, the response-time of this kind of sensors is typically slow because reagents ...

A comprehensive numerical treatment of the diffusion and reaction within a sandwich-type amperometric biosensor is presented. The model considers that the enzyme reacts according to a ping-pong mechanism and that it is ...

A mathematical model to describe the amperometric response of a sandwich-type biosensor containing polyphenol oxidase as the recognition catalytic element is proposed. The model was solved numerically and the resulting ...

Software for the automatic non-linear least squares fit of chronoamperometric responses corresponding to sandwich-type amperometric biosensors has been developed. The so-called Simplex algorithm computes a minimum value ...

In the past decades, great effort has been put in finding new electrode surface modifiers and enzyme immobilizer agents that prevent the enzyme leakage, minimize the effect of interfering species, retain the enzyme ...

This work reports an amperometric enzyme-electrode prepared with glucose oxidase, which have been immobilized by a cross-linking step with glutaraldehyde in a mixture containing albumin and a novel carbon nanotubes-mucin ...

We present an electrochemical sandwich-type assay based on the splitting of an aptamer into two fragments. Gold nanoparticles are modified with one of the fragments and a redox polyelectrolyte. The first is used as the ...