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Production of recombinant enzymes of wide use for research
(Pontificia Universidad Católica de Valparaíso, 2006)
Cloning, overexpression, and purification of functional human purine nucleoside phosphorylase
(2003-01-01)
Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. A genetic deficiency due to mutations in the gene encoding for human PNP causes T-cell ...
Multi-compartment and multi-host vector suite for recombinant protein expression and purification
(Frontiers Media, 2018)
Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous ...
Aqueous Biphasic Systems Composed of Cholinium Chloride and Polymers as Effective Platforms for the Purification of Recombinant Green Fluorescent Protein
(2018-07-02)
Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker; however, its widespread use is limited by its purification costs. Alternative low-cost purification techniques can overcome this issue. ...
Affinity purification of recombinant proteins using a LysM domain and bacterium like particles
(Elsevier Science, 2018-08)
The lysin motif (LysM) is a ubiquitous motif across kingdoms, which in bacteria allows cell wall degrading enzymes to bind noncovalently to peptidoglycan. This property has been exploited for two decades to design mucosal ...
Human interferon B1 ser17: Coding DNA synthesis, expression, purification and characterization of bioactive recombinant protein
(2010-09-01)
A protocol to produce large amounts of bioactive homogeneous human interferon β1 expressed in Escherichia coli was developed. Human interferon β1 ser17 gene was constructed, cloned and subcloned, and the recombinant protein ...
Human interferon B1 ser17: Coding DNA synthesis, expression, purification and characterization of bioactive recombinant protein
(2010-09-01)
A protocol to produce large amounts of bioactive homogeneous human interferon β1 expressed in Escherichia coli was developed. Human interferon β1 ser17 gene was constructed, cloned and subcloned, and the recombinant protein ...
Expression and purification of human respiratory syncytial virus recombinant fusion protein
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2008)
The Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The ...