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Quantification of bovine leukemia virus proviral DNA using a low-cost real-time polymerase chain reaction
(Elsevier, 2018-07)
The detection of bovine leukemia virus (BLV) proviral DNA is an important tool to address whether an animal is infected with BLV. Compared with serological assays, real-time PCR accounts for greater sensitivity and can ...
Improved DNA extraction technique from clot for the diagnosis of Chagas disease
(Public Library of Science, 2019)
BACKGROUND: The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater ...
Comparative gene expression analysis throughout the life cycle of Leishmania braziliensis: Diversity of expression profiles among clinical isolates
(Public Library of Science, 2011)
Background: Most of the Leishmania genome is reported to be constitutively expressed during the life cycle of the parasite, with a few regulated genes. Inter-species comparative transcriptomics evidenced a low number of ...
Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
(Elsevier, 2015)
An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and ...
Real-time PCR assay for detection and quantification of Leishmania (Viannia) organisms in skin and mucosal lesions: Exploratory study of parasite load and clinical parameters
(American Society for Microbiology, 2013)
Earlier histopathology studies suggest that parasite loads may differ between cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML) lesions and between acute and chronic CL. Formal demonstration requires highly ...
Regional Differences of Leptospirosis in Sri Lanka: Observations from a Flood-Associated Outbreak in 2011
(Public Library of Science, 2014)
Leptospirosis is known to be an important cause of weather disaster-related infectious disease epidemics. In 2011, an outbreak of leptospirosis occurred in the relatively dry district of Anuradhapura, Sri Lanka where ...
Multiplex real-time PCR for detection of Campylobacter, Salmonella, and Shigella
(American Society for Microbiology, 2013)
Infectious diarrhea can be classified based on its clinical presentation as noninflammatory or inflammatory disease. In developing countries, among inflammatory diarrhea cases, Shigella is the most common cause, followed ...