Dengue virus is an important human pathogen that belongs to the Flaviviridae family. The viral genome is a single molecule of RNA of positive polarity that plays multiple roles during the viral life cycle. Besides encoding ...

The mechanism by which viral RNA-dependent RNA polymerases (RdRp) specifically amplify viral genomes is still unclear. In the case of flaviviruses, a model has been proposed that involves the recognition of an RNA element ...

Long-range and local RNA-RNA contacts in viral RNA genomes result in tertiary structures that modulate the function of enhancers, promoters, and silencers during translation, RNA replication, and encapsidation. In the case ...

Transfer RNA-dependent amidotransferases (AdTs) are essential for Gln-tRNA Gln and Asn-tRNA Asn synthesis in organisms lacking glutaminyl-or asparaginyl-tRNA synthetase. In the presence of ATP and an amide donor, AdT ...

Flavivirus genome amplification is a complex process that involves the viral RNA, cellular and viral proteins, and a sophisticated architecture of cellular membranes induced by viral infection. The viral RNA is not just a ...

Microinjection of poliovirus RNA in Xenopus oocytes initiates a complete and authentic viral replication cycle that yields newly synthesized infectious virus. This system can be used to study the molecular mechanism of the ...

Dengue virus RNA-dependent RNA polymerase specifically binds to the viral genome by interacting with a promoter element known as stem-loop A (SLA). Although a great deal has been learned in recent years about the function ...