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RNA extraction from the yeast candida parapsilosis sensu stricto using two commercially available silica column-based purification methods
(2018)
Good quality ribonucleic acid (RNA) needs to be obtained in order to study gene expression. Different RNA extraction methods have been described, but RNA quality and yield may vary among the different techniques and ...
RNA extraction from the yeast candida parapsilosis sensu stricto using two commercial methods based on purification by silica columns
(2018)
Good quality RNA needs to be obtained in order to study gene expression. Different RNA extraction methods have been described, but RNA quality and yield may vary among the different techniques and biological study species. ...
Identifying trypanosome protein-RNA interactions using RIP-Seq
(Humana Press, 2020)
Trypanosomatids rely primarily on post-transcriptional mechanisms for the control of gene expression, with regulation of RNA processing, localization, degradation and translation by RNA-binding proteins (RBPs). To determine ...
Comparison of commercially-available RNA extraction methods for effective bacterial RNA isolation from milk spiked samples
(Pontificia Universidad Católica de Valparaíso, 2010)
Dual color fluorescence in situ hybridization (FISH) assays for detecting Mycobacterium tuberculosis and Mycobacterium avium complexes and related pathogens in cultures
(Public Library of Science, 2017)
Two rapid dual color fluorescence in situ hybridization (FISH) assays were evaluated for detecting M. tuberculosis and related pathogens in cultures. The MN Genus-MTBC FISH assay uses an orange fluorescent probe specific ...
Production and characterization of a highly pure RNA polymerase holoenzyme from Mycobacterium tuberculosis
(Elsevier, 2017)
Recent publications have shown that active RNA polymerase (RNAP) from Mycobacterium tuberculosis (MtbRNAP) can be produced by expressing all four subunits in a single recombinant Escherichia coli strain [1-3]. By reducing ...
Occurrence of Giardia intestinalis and Cryptosporidium sp in wastewater samples from Sao Paulo State, Brazil, and Lima, Peru
(Springer, 2016)
The objectives of the study were to detect and genotype Cryptosporidium spp. and Giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the State of Sao Paulo, Brazil, and at ...
Purification of Exosomes from Primary Schwann Cells, RNA Extraction, and Next-Generation Sequencing of Exosomal RNAs
(2018)
Exosomes are small (30-150 nm) vesicles of endosomal origin secreted by most cell types. Exosomes contain proteins, lipids, and RNA species including microRNA, mRNA, rRNA, and long noncoding RNAs. The mechanisms associated ...
Extraction and purification of total RNA from banana tissues (small scale)
(CAMBRIDGE UNIV PRESS, 2008)
Introduction. This protocol aims at preparing total RNA for gene expression analysis by Northern blots, RT-PCR and real-time quantitative PCR; cDNA isolation by RTPCR; and cDNA library construction. The principle, key ...