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RNA extraction from the yeast candida parapsilosis sensu stricto using two commercially available silica column-based purification methods
(2018)
Good quality ribonucleic acid (RNA) needs to be obtained in order to study gene expression. Different RNA extraction methods have been described, but RNA quality and yield may vary among the different techniques and ...
RNA extraction from the yeast candida parapsilosis sensu stricto using two commercial methods based on purification by silica columns
(2018)
Good quality RNA needs to be obtained in order to study gene expression. Different RNA extraction methods have been described, but RNA quality and yield may vary among the different techniques and biological study species. ...
A micromethod for high throughput RNA extraction in forest trees
(Sociedad de Biología de Chile, 2007)
Comparison of commercially-available RNA extraction methods for effective bacterial RNA isolation from milk spiked samples
(Pontificia Universidad Católica de Valparaíso, 2010)
Storage of bovine reproductive tissues and RNA extracts on ice for 24h or repeated freeze–thaw cycles do not affect RNA integrity
(European Society for Domestic Animal Reproduction (ESDAR)European Veterinary Society of Small Animal Reproduction (EVSSAR)Spanish Society of Animal ReproductionMalden, 2014-02)
The aims of this study were to test (i) the effect of time of tissue and RNA extracts storage on ice and (ii) the effect of repeated freeze–thaw cycles on RNA integrity and gene expression of bovine reproductive tissues. ...
Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis
(Brazilian Archives of Biology and Technology, 2011-10-01)
The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 ...
Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis
(Brazilian Archives of Biology and Technology, 2011-10-01)
The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 ...
Extraction and purification of total RNA from banana tissues (small scale)
(CAMBRIDGE UNIV PRESS, 2008)
Introduction. This protocol aims at preparing total RNA for gene expression analysis by Northern blots, RT-PCR and real-time quantitative PCR; cDNA isolation by RTPCR; and cDNA library construction. The principle, key ...
Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis
(Brazilian Archives of Biology and Technology, 2014)
Isolation of high quality RNA from soil-grown Ilex paraguariensis roots suitable for next-generation sequencing and gene expression analyses
(Southern Cross Publ, 2017-07)
Extraction of high quality RNA is a prerequisite for downstream application in functional genomics analyses. However, the extraction and purification of pure nucleic acids from root tissues is generally difficult due to ...