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Validation of reference genes for gene expression studies in the honey bee, Apis mellifera, by quantitative real-time RT-PCR
(SPRINGER FRANCE, 2008)
For obtaining accurate and reliable gene expression results it is essential that quantitative real-time RT-PCR (qRT-PCR) data are normalized with appropriate reference genes. The current exponential increase in postgenomic ...
Transcriptional expression analysis of oestrogen receptor in ovary of Texel and Araucana sheep breeds by real time quantitative RT-PCRAnálisis de la expresión transcripcional del receptor de estrógeno en ovario de ovejas prepúberes de razas Texel y Criolla Araucana mediante RT-PCR cuantitativo en tiempo real
(Archivos de Medicina Veterinaria, 2015)
Specific Detection of Bovine Coronavirus N Protein with TaqMan Probe qRT-PCR
(Universidade Federal do Rio Grande do Sul, 2019)
Detecção do vírus da cinomose canina por diferentes métodos de one-step RT-qPCR
(2016-09-01)
Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and ...
Reference gene selection for quantitative PCR in liver, skeletal muscle, and jejunum of Bos indicus cattle
(Sociedade Brasileira de Zootecnia (SBZ), 2022)
Evaluation of Quantitative RT-PCR Using Nonamplified and Amplified RNA
(LIPPINCOTT WILLIAMS & WILKINS, 2010)
Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is a standard assay in molecular medicine for gene expression analysis. Samples from incisional/needle biopsies, laser-microdissected tumor cells and ...
Characterization of specific cDNA background synthesis introduced by reverse transcription in RT-PCR assays
(Elsevier France-editions Scientifiques Medicales Elsevier, 2010-12)
To block expression of NMDA receptor NR1 subunit, we injected into rat hippocampus a Herpes Simplex Virus type 1 derived vector bearing a sequence for NR1 antisense. RT-PCR assays with RNA from hippocampus of animals ...
A Sigmoidal Model for the Interpretation of Quantitative PCR (QPCR) Experiments
(Universidad Militar Nueva Granada, 2016)
Quantitative real-time RT-PCR and chromogenic in situ hybridization: precise methods to detect HER-2 status in breast carcinoma
(Biomed Central Ltd., 2009-03-23)
Background: HER-2 gene testing has become an integral part of breast cancer patient diagnosis. The most commonly used assay in the clinical setting for evaluating HER-2 status is immunohistochemistry (IHC) and fluorescence ...