In this work, the influence of the C 12 -, C 14 -, and C 16 -alkyl chain derivatives of 1-alkyl-3-methyl-imidazole tetrafluoroborate over the hydrolisis of p-nitrophenyl trimethylacetate catalyzed by α-chymotrypsin was ...

During the last 60 years, aqueous two-phase systems (ATPS) have been the focus of much research. A large number of studies have been reported on the characterization of these systems and their use on separation, analytic, ...

Over the last few years, researchers have started to explore a particular class of compounds defined as ionic liquids (ILs) in attempts to use their unique characteristics. Since ILs have a very low vapor pressure, these ...

Using molecular dynamics simulations, we investigated the thermal stability and real-time denaturation of a model mini-protein in four solvents: (1) water, (2) 1-ethyl-3-methylimidazolium alaninate [EMIM][ALA] (5 mol% in ...

In this work, the influence of the C12-, C14-, and C16-alkyl chain derivatives of 1-alkyl-3-methyl-imidazole tetrafluoroborate over the hydrolisis of p-nitrophenyl trimethylacetate catalyzed by α-chymotrypsin was studied ...

Fluorescent Proteins (FP) can be applied as biomarkers and biosensors in the industrial and medical fields, but their large-scale use, especially for new industrial applications, is limited due to their low stability. ...

Aqueous micellar two-phase systems (AMTPS) using surfactants have been widely applied as an advantageous extraction technology for a wide range of (bio)molecules. When composed by two surfactants (the second one used as ...

Green fluorescent protein (GFP) is a useful biomolecule in biotechnology; however, its price makes its widespread application prohibitive. To overcome this issue, recently, the use of aqueous two-phase systems (ATPS) for ...