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Development and comparative analysis of yeast protein extraction protocols for mass spectrometry
(2019-02-15)
Mass spectrometry is the most used method for protein identification and quantification. Here we developed four protein extraction protocols precisely for mass spectrometry, and we compared with other ones already published. ...
Two-dimensional gel electrophoresis analysis of brown algal protein extracts
(2008)
High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus ...
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
(Universidad Nacional de Colombia - Sede Palmira, 2017-04-01)
Cocoa, Theobroma cacao L. is one of the main tropical industrial crops. Cocoa has a very high level of interfering substances, such as polysaccharides and phenolic compounds that could prevent the isolation of suitable ...
Method optimization for proteomic analysis of soybean leaf: improvements in identification of new and low-abundance proteins
(Genetics and Molecular Biology, 2017)
Evaluation of sample preparation protocols for proteomic analysis of sunflower leaves
(Elsevier Science BvAmsterdamHolanda, 2010)
Sample preparation focusing on plant proteomics: extraction, evaluation and identification of proteins from sunflower seeds
(Royal Soc ChemistryCambridgeInglaterra, 2013)
NMR-based identification of metabolites in polar and non-polar extracts of avian liver
(MDPI, 2017-12)
Metabolites present in liver provide important clues regarding the physiological state of an organism. The aim of this work was to evaluate a protocol for high-throughput NMR-based analysis of polar and non-polar metabolites ...
Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments
(2018)
Background: The Plasmodium vivax Duffy binding protein (PvDBP) has been the most studied ligand binding human reticulocytes to date. This molecule has a cysteine-rich domain in region II (RII) which has been used as control ...