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Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding
(Asociación Argentina de Microbiología, 2016-01)
Bacillus anthracis protective antigen (PA) is a well known and relevant immunogenicprotein that is the basis for both anthrax vaccines and diagnostic methods. Properly foldedantigenic PA is necessary for these applications. ...
Heterologous expression, purification and refolding of an anti-listerial peptide produced by Pediococcus acidilactici K7
(Pontificia Universidad Católica de Valparaíso, 2007)
Production of the refolded oligopeptide-binding protein (OppA) encoded by the citrus pathogen Xanthomonas axonopodis pv. citri
(FUNPEC-EDITORA, 2008)
The oligopeptide-binding protein, OppA, binds and ushers oligopeptide substrates to the membrane-associated oligopeptide permease (Opp), a multi-component ABC-type transporter involved in the uptake of oligopeptides expressed ...
Protein refolding at high pressure: Optimization using eGFP as a model
(Elsevier Sci LtdOxfordInglaterra, 2011)
Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli
(Pontificia Universidad Católica de Valparaíso, 2011)
DNA and heparin chaperone the refolding of purified recombinant replication protein A subunit 1 from Leishmania amazonensis
(Elsevier B.V., 2009-02-01)
Replication protein A (RPA) is a single-stranded DNA-binding protein that has been implicated in DNA metabolism and telomere maintenance. Subunit 1 of RPA from Leishmania amazonensis (LaRPA-1) has previously been ...
DNA and heparin chaperone the refolding of purified recombinant replication protein A subunit 1 from Leishmania amazonensis
(Elsevier B.V., 2009-02-01)
Replication protein A (RPA) is a single-stranded DNA-binding protein that has been implicated in DNA metabolism and telomere maintenance. Subunit 1 of RPA from Leishmania amazonensis (LaRPA-1) has previously been ...
Investigation on solubilization protocols in the refolding of the thioredoxin TsnC from Xylella fastidiosa by high hydrostatic pressure approach
(ElsevierMaryland Heights, 2015-02)
The lack of efficient refolding methodologies must be overcome to take full advantage of the fact that bacteria express high levels of aggregated recombinant proteins. High hydrostatic pressure (HHP) impairs intermolecular ...