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Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
(National Research Council Canada-NRC Research Press, 2014-12)
The aim of this study was to assess the effects of agronomic practices on arbuscular mycorrhizal (AM) fungal community in soils from the Pampa Ondulada region (Argentina), and to compare conclusions reached when using ...
Development of an ELA-DRA gene typing method based on pyrosequencing technology
(Wiley Blackwell Publishing, Inc, 2008-11)
The polymorphism of equine lymphocyte antigen (ELA) class II DRA gene had been detected by polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) and reference strand-mediated conformation analysis. ...
Development of typing methods based on pyrosequencing technology fot the analysis of six bovine genes related to marbling
(Sociedad Argentina de Genética, 2013-12)
Several methods such as PCR-RFLP, OLA, DNA sequencing, PCR-SSCP and ARMS-PCR have been developed to detect the allelic variation at substitutions K232A of DGAT1, GH6.1 of GH, F279Y of GHR, R4C of LEP, I74V of FABP4, and ...
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare
(2016-07-01)
Pressmud is a substrate derived from sugarcane juice filtrate, and around 26–40 kg of this residue are produced per ton of sugarcane. It is mainly used as fertilizer in crops, and its application in the field is often made ...
Microsatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technology
(Springer, 2015-06-01)
Among the pelagic shark species most impacted by fisheries, the oceanic whitetip shark (Carcharhinus longimanus) has shown signs of population declines. In this study, we developed 12 microsatellite markers using next ...
Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology
(Peerj Inc, 2016-08-30)
The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the ...
Two amplicon sequencing strategies revealed different facets of the prokaryotic community associated with the anaerobic treatment of vinasses from ethanol distilleries
(Elsevier, 2014-02)
The prokaryotic consortium from a pilot-scale UASB reactor fed with vinasses from ethanol distilleries was evaluated by means of amplicon sequencing of the 16S rRNA gene. Two different sets of primers targeted to overlapping ...
High sensitivity of 454 pyrosequencing for detection of rare species in aquatic communities
(Wiley, 2013-06)
1. Concerns regarding the rapid loss of endemic biodiversity, and introduction and spread of non-indigenous species, have focused attention on the need and ability to detect species present in communities at low abundance. ...