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Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities
(2022)
The Cas1 protein is essential for the functioning of CRISPR-Cas adaptive systems. However, despite the high prevalence of CRISPR-Cas systems in thermophilic microorganisms, few studies have investigated the occurrence and ...
Application of crispr/cas9-based reverse genetics in leishmania braziliensis: Conserved roles for hsp100 and hsp23
(MDPI, 2020)
The protozoan parasite Leishmania (Viannia) braziliensis (L. braziliensis) is the main cause of human tegumentary leishmaniasis in the New World, a disease affecting the skin and/or mucosal tissues. Despite its importance, ...
Application of crispr/cas9-based reverse genetics in leishmania braziliensis: Conserved roles for hsp100 and hsp23
(MDPI AG, 2020-10-01)
The protozoan parasite Leishmania (Viannia) braziliensis (L. braziliensis) is the main cause of human tegumentary leishmaniasis in the New World, a disease affecting the skin and/or mucosal tissues. Despite its importance, ...
Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing
(John Wiley & Sons Inc, 2019-08)
The clustered regularly interspaced short palindromic repeats (CRISPR) system?associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored ...
Casein and whey protein in the breast milk ratio: Could it promote protein metabolism enhancement in physically active adults?
(2021-07-01)
Due to the utilization of milk proteins such as whey protein (WP) and casein as sports nutrition ergogenic aids, the present study investigated the effects of the association of WP and casein in a ratio of 80:20, a similar ...
Characterising ISWI chromatin remodeler in Trypanosoma cruzi
(Fundação Oswaldo Cruz. Instituto Oswaldo Cruz, 2020)
Produção de células MDBK expressando a enzima CAS9 e edição do gene da beta-lactoglobulina pelo sistema CRISPR/Cas9
(Universidade Federal de Juiz de Fora (UFJF)BrasilICB – Instituto de Ciências BiológicasPrograma de Pós-graduação em Ciências Biológicas: Imunologia e Doenças Infecto-Parasitárias/Genética e BiotecnologiaUFJF, 2018)