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Living Cell Manipulation, Manageable Sampling, and Shotgun Picoliter Electrospray Mass Spectrometry for Profiling Metabolites
(Elsevier, 2013-01)
A modified cell pressure probe and an online Orbitrap mass spectrometer were used to sample in situ plant single cells without any additional manipulation. The cell pressure probe, a quartz capillary tip filled with an oil ...
Integrative Analysis of Physiological Phenotype of Plant Cells by Turgor Measurement and Metabolomics
(International Association of Engineers, 2012-12)
Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling "in situ" living plant cell status, turgor pressure of cells located at different locations ...
Single-cell metabolite profiling of stalk and glandular cells of intact trichomes with internal electrode capillary pressure probe electrospray ionization mass spectrometry
(American Chemical Society, 2016-02)
In this report, we developed the pressure probe electrospray ionization-mass spectrometry with internal electrode capillary (IEC-PPESI-MS) which enables high spatial-resolution cell sampling, precise postsampling manipulation, ...
On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
(Nature, 2020-12)
Although a loss of healthy pollen grains induced by metabolic heat responses has been indicated to be a major cause of heat-induced spikelet sterility under global climate change, to date detailed information at pollen ...