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Proacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa

Cortes, Constanza J.; Codelia, Veronica A.; Manosalva, Iris; de Lange, Johanna; De los Reyes, Monica; Moreno, Ricardo D. (ELSEVIER, 2006)

The scope of the present study was to evaluate the presence and activation of proacrosin/acrosin as a tool to determine the acrosomal status of fresh and frozen/thawed dog spermatozoa. Monoclonal antibody C5F11, directed ...

Improved islet functional recovery, survival, and preservation of engraftment capacities post-thawing using a CGMP vitrification system for subsequent islet transplantationAdvanced Cell-Based and Regenerative Therapies in Mexico.

Robles Murillo, Ana Karina; Najar Acosta, Luis Jesús; Estrella Villaseñor, Heriberto Amador; Zepeda Moreno, Abraham; Sanchez Zubieta, Fernando Antonio; Sánchez Enríquez, Sergio; Villafán Bernal, José Rafael; Rivas Carrillo, Jorge David (Wolters Kluwer Health, Inc., 2022)

Does Cryopreservation Affect the Biological Properties of Stem Cells from Dental Tissues? A Systematic Review

Conde, Marcus Cristian Muniz; Chisini, Luiz Alexandre; Grazioli, Guillermo; Francia, Alejandro; Carvalho, Rodrigo Varella de; Alcázar, Jose Carlos Bernedo; Tarquinio, Sandra Beatriz Chavez; Demarco, Flavio Fernando (Fundação Odontológica de Ribeirão Preto, 2016)

This systematic review evaluated if different cryopreservation protocols could affect biological properties (Cell survival rate (CSR), proliferation, differentiation, maintenance of stem cell markers) of stem cells obtained ...

A comparison of post-thaw results between embryos arising from intracytoplasmic sperm injection using surgically retrieved or ejaculated spermatozoa

FIGUEIRA, Rita de Cassia Savio; MADASCHI, Camila; NICHI, Marcilio; RODRIGUES, Debora; PASQUALOTTO, Fabio Firmbach; IACONELLI JR., Assumpto; BORGES, Edson (ELSEVIER SCIENCE INC, 2009)

Objective: To study the effect of freeze-thaw on embryos derived from intracytoplasmic sperm injection (ICSI) using surgically retrieved and ejaculated spermatozoa. Design: Retrospective study. Setting: Private IVF center. ...

Improving Cell Recovery: Freezing and Thawing Optimization of Induced Pluripotent Stem Cells

Uhrig, Markus; Ezquer, Fernando; Ezquer, Marcelo (2022)

Achieving good cell recovery after cryopreservation is an essential process when working with induced pluripotent stem cells (iPSC). Optimized freezing and thawing methods are required for good cell attachment and survival. ...

Estudo dos subtipos celulares do sangue de cordão umbilical e sua viabilidade antes do processamento e após o descongelamento = armazenamento à temperatura ambiente por 96 horas =Study of umbilical cord blood cell subtypes and its viability prefreezing and post-thaw: stored pre-freezing at room temperature for 96 hoursStudy of umbilical cord blood cell subtypes and its viability prefreezing and post-thaw : stored pre-freezing at room temperature for 96 hours

Pereira-Cunha, Fernanda Gonçalves, 1968- ([s.n.], 2014)

Effect of royal jelly on bovine sperm characteristics during post–thaw incubation in vitroEfectos de la incubación in vitro con jalea real sobre las características del semen bovino descongelado

Abd–Allah, Saber Mohammed; Department of Theriogenology, Faculty of Veterinary Medicine, Beni–Suef University, 62511 Beni–Suef (Universidad Nacional del Nordeste, 2010)

Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry

LEITE, Ticiano Guimarães; VALE FILHO, Vicente Ribeiro do; Arruda, Rubens Paes de; Andrade, André Furugen Cesar de; EMERICK, Lucas Luz; Zaffalon, Fabiane Gilli; MARTINS, Jorge André Matias; ANDRADE, Venício José de (Amsterdam, 2010)

The objectives of the present study were to investigate the effects of three equilibration times (0, 2, and 4 h) and two extenders (TRIS or Bioxcell®) for cryopreservation of bull semen. Semen from 12 Gyr bulls was ...

Cryoprotective effect of different glycerol concentrations on domestic cat spermatozoa

Universidade Estadual Paulista (Unesp); Fundação Amaral Carvalho (2013-10-15)

Cryopreservation of spermatozoa is a pivotal tool in assisted reproduction, and studies aiming to establish optimal freezing/thawing protocols are essential to enhance sperm survival. The objectives of the present study ...

Two-step accelerating freezing protocol yields a better motility, membranes and DNA integrities of thawed ram sperm than three-steps freezing protocols

Galarza Lucero, Diego Andres; López Sebastián, Antonio; Woelders, Henri; Blesbois, Elizabeth; Santiago Moreno, Julián (2019)

The present study compares a protocol that mimics freezing of ram semen in static nitrogen vapor with two protocols with an initial low cooling rate in the first step, followed by higher cooling rates where ice nucleation ...

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