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Variably improved microbial source tracking with digital droplet PCR
(Pergamon-Elsevier Science Ltd, 2019-08-01)
This study addressed whether digital droplet PCR (ddPCR) could improve sensitivity and specificity of human-associated Bacteroidales genetic markers, BacHum and B. theta, and their quantification in environmental and fecal ...
Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A
(Univ Federal Santa MariaSanta MariaBrasil, 2009)
Optimization of Methods for Detecting Hepatitis A Virus in Food
(Springer Verlag, 2017)
Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of canine ehrlichiosis
(Colégio Brasileiro de Parasitologia Veterinária, 2010-06-01)
O objetivo deste estudo foi aperfeiçoar um ensaio de PCR que amplificasse um fragmento de 843 pares de bases do gene p28 da Ehrlichia canis e compará-lo com outros dois métodos de PCR utilizados para amplificar partes do ...
Characterization of human intestinal bifidobacteria using competitive PCR and PCR-TTGE
(BLACKWELL, 2006-01)
In this study, a competitive PCR was developed to estimate the quantity of bifidobacteria in human faecal samples using two 16S rRNA gene Bifidobacterium genus-specific primers, Bif164f and Bif662r. A PCR-temporal temperature ...
Malaria diagnosis from pooled blood samples: comparative analysis of real-time PCR, nested PCR and immunoassay as a platform for the molecular and serological diagnosis of malaria on a large-scale
(Instituto Oswaldo Cruz, Ministério da Saúde, 2011)
Malaria diagnoses has traditionally been made using thick blood smears, but more sensitive and faster techniques are required to process large numbers of samples in clinical and epidemiological studies and in blood donor ...
Desenvolvimento de PCR convencional e em tempo real para o Vírus da Língua Azul
(Universidade Federal de Minas GeraisUFMG, 2010-11-05)
The Bluetongue (BT) is a non-contagious infectious disease that has important socio-economic impacts of restrictions on import and / or by direct losses in cattle infected with bluetongue virus (BTV). The BTV is a member ...
Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples
(2008-12-01)
Background. The use of methods, both sensitive and specific, for rabies diagnosis are important tools for the control and prophylaxis of the disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used ...
Analytical performance of Four Polymerase Chain Reaction (PCR) and real time PCR (qPCR) assays for the detection of six Leishmania species DNA in Colombia
Leishmaniasis comprises a spectrum of parasitic diseases caused by protozoans of the genus Leishmania. Molecular tools have been widely employed for the detection of Leishmania due to its high sensitivity and specificity. ...