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Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65 patterns
(2008)
Abstract
Background
Identification of nontuberculous mycobacteria (NTM) based on phenotypic tests is time-consuming, ...
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
(Blackwell Publishing, 2007-04-01)
Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, ...
Evaluation of hsp65 Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country
(Hindawi Publishing Corporation, 2013-01-01)
Current study evaluated the hsp65 Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65 Nested PCR-PRA) to detect and identify Mycobacterium tuberculosis complex directly in clinical samples for a rapid and ...
Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
(2010)
The presence of environmental mycobacteria on surfaces in two public health institutions, namely a health center and a hospital in upstate São Paulo (Brazil), was identified by polymerase chain reaction-restriction enzyme ...
Use of the polymerase chain reaction to detect Mycobacterium leprae in urine
(ASSOC BRAS DIVULG CIENTIFICASAO PAULO, 2012)
Leprosy is an infectious disease caused by Mycobacterium leprae. The polymerase chain reaction (PCR) has been applied to detect M. leprae in different clinical samples and urine seems to be attractive for this purpose. PCR ...
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil
(Sociedade Brasileira de Microbiologia, 2014-06-01)
Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed ...
Isolamento microbiológico e caracterização molecular de micobactérias no leite de vacas positivas no teste de tuberculinização cervical comparativoMicrobiological and molecular identification of mycobacteria in milk from cows positive to the cervical comparative tuberculin test
(Universidade Estadual Paulista (Unesp), 2016)
Identificación de micobacterias no tuberculosas (MNT) o atípicas por medio de la técnica de diagnóstico molecular polymerase chain reaction (PCR) and restriction enzime analisis (PRA) and restriction enzyme analysis (PRA), analisis de patrones de restricción
(ESPE / SANGOLQUÍ / 2008, 2008)
Las micobacterias atípicas son un grupo heterogéneo de microorganismos (saprofitas, patógenas u oportunistas) distribuidos ampliamente en la naturaleza. Este grupo comprende a todas las micobacterias a excepción del complejo ...
hsp65 PCR-restriction enzyme analysis (PRA) for identification of mycobacteria in the clinical laboratory
(Instituto de Medicina Tropical, 2001-02-01)
More than 70 species of mycobacteria have been defined, and some can cause disease in humans, especially in immunocompromised patients. Species identification in most clinical laboratories is based on phenotypic characteristics ...
Allele Frequency of the C.5G>A Mutation in the PRCD Gene Responsible for Progressive Retinal Atrophy in English Cocker Spaniel Dogs
(2019-10-01)
Progressive retinal atrophy (PRA) due to the c.5G>A mutation in the progressive rod-cone degeneration (PRCD) gene is an important genetic disease in English cocker spaniel (ECS) dogs. Because the prevalence of this disease ...