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Avaliação de suportes hidrofóbicos aplicados à purificação de enzimas
(Universidade Federal de São CarlosUFSCarPrograma de Pós-Graduação em Engenharia Química - PPGEQCâmpus São Carlos, 2019-02-28)
Enzymes are biological catalysts that present diverse applications, representing interesting perspectives for use in several branches of the industry: agribusiness, biofuels, and pharmacist. However, the recovery of broth ...
Identificação de proteínas antimicrobianas de flores de alecrim-pimenta (Lippia sidoides): uma nova estratégia no combate a patógenos
(Universidade Federal de Juiz de Fora (UFJF)BrasilICB – Instituto de Ciências BiológicasPrograma de Pós-graduação em Ciências Biológicas: Imunologia e Doenças Infecto-Parasitárias/Genética e BiotecnologiaUFJF, 2016)
Microbial lipase: a new approach for a heterogeneous biocatalyst
(2020-01-01)
Lipases are enzymes employed in several industrial process and their applicability can be increased if these biocatalysts are in the immobilize form. The objective of this work was to study the immobilization of lipase ...
Biochemical properties of free and immobilized Candida viswanathii lipase on octyl-agarose support: Hydrolysis of triacylglycerol and soy lecithin
(2018-02-01)
Microbial lipases are important enzymes in food and pharmaceutical industries. In this work, a Candida viswanathii lipase was purified by hydrophobic interaction chromatography on octyl Sepharose. The purification presented ...
Selectivity of R-alpha-monobenzoate glycerol synthesis catalyzed by Candida antarctica lipase B immobilized on heterofunctional supports
(2015)
The main goal of this work was to study different strategies of immobilization of Candida antarctica lipase B (CALB) in order to evaluate changes in the selectivity of this enzyme when R-alpha-monobenzoate glycerol ...
Immobilization of lipase from Penicillium sp. section Gracilenta (CBMAI 1583) on different hydrophobic supports: Modulation of functional properties
(2017-01-01)
Lipases are promising enzymes that catalyze the hydrolysis of triacylglycerol ester bonds at the oil/water interface. Apart from allowing biocatalyst reuse, immobilization can also affect enzyme structure consequently ...
Hydrophobic interaction chromatography for purification of monoPEGylated RNase A
(ELSEVIER SCIENCE BV, 2012-06-15)
The chromatographic methods used for the purification of PEGylated proteins are mainly Size Exclusion
(SEC) and Ion Exchange Chromatography (IEX). Although the PEGylation affects the protein hydrophobicity,
Hydrophobic ...
Resposta imune celular no melanoma murino: identificação de componentes tumorais envolvidos na resposta funcional de macrófagos
(Universidade Federal de São Paulo (UNIFESP), 2002)
Componentes de membrana do melanoma murino B16F10-NEX2 foram capazes de ativar macrofagos peritoneais de camundongos C3H/HeJ e C57BL/6 para a producao de IL-12 e NO. Esses componentes foram obtidos por sonicacao das celulas, ...