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Design of affinity chromatography peptide ligands through combinatorial peptide libraries screening
(Springer, 2020)
In this chapter, a protocol to design affinity chromatography matrices with short peptide ligands immobilized for protein purification is described. The first step consists of the synthesis of a combinatorial peptide library ...
Peptide affinity chromatography based on combinatorial strategies for protein purification
(Humana Press, 2014)
We describe a method to develop affinity chromatography matrices with short peptide ligands for protein purification. The method entitles the following: a) synthesis of a combinatorial library on the HMBA-ChemMatrix resin ...
Friendly Strategy to Prepare Encoded One Bead−One Compound Cyclic Peptide Library
(ACS Publications, 2013-09-06)
One bead−one peptide libraries allow the screening of suitable ligands for any target protein. Short cyclic peptides are ideal ligands for affinity chromatography because of their high affinity and selectivity for the ...
Protein purification by affinity chromatography with peptide ligands selected from the screening of combinatorial libraries
(Research Trends, 2010-12)
Affinity chromatography is likely to play an everincreasing important role in protein purification as it is the most effective method for the direct isolation and purification of biomolecules from complex mixtures. Successful ...
Two-stage screening of combinatorial peptide libraries: Application to bovine serum albumin ligand selectionDoble screening de bibliotecas combinatorias peptídicas: Su aplicación en la selección de ligandos para la seroalbúmina bovina
(Centro Nacional de Investigaciones Científicas, 2015-04)
Los péptidos cortos son excelentes ligandos para cromatografía de afinidad. El método dividir-acoplar-recombinar permite obtener bibliotecas peptídicas con todas las combinaciones de los aminoácidos en la forma de “una ...
Combinatorial Library Screening Coupled to Mass Spectrometry toIdentify Valuable Cyclic Peptides
(Wiley, 2016-01)
Combinatorial library screening coupled to mass spectrometry (MS) analysis isa practical approach to identify useful peptides. Cyclic peptides can have highbiological activity, selectivity, and affinity for target proteins, ...
Fully automated screening of a combinatorial library to avoid false positives: application to tetanus toxoid ligand identification
(American Chemical Society, 2021-07)
Peptide ligands are widely used in protein purification by affinity chromatography. Here, we applied a fully automated two-stage library screening method that avoids false positive peptidyl-bead selection and applied it ...
Latest Advances in OBOC Peptide Libraries: Improvements in Screening Strategies and Enlarging the Family From Linear to Cyclic Libraries
(Bentham Science Publishers, 2016-03)
Solid phase screenings of one bead one compound (OBOC) libraries have been widely used to find ligands with pharmacological and analytical uses, and to purify or detect proteins in complex mixtures. To improve library ...
Correlation between the mobility of spin-labeled peptide chains and resin solvation: An approach to optimize the synthesis of aggregating sequences
(Amer Chemical Soc, 1999-12-10)
Resin solvation properties affect the efficiency of the coupling reactions in solid-phase peptide synthesis. Here we report a novel approach to evaluate resin solvation properties, making use of spin label electron ...
Correlation between the mobility of spin-labeled peptide chains and resin solvation: An approach to optimize the synthesis of aggregating sequences
(Amer Chemical Soc, 1999-12-10)
Resin solvation properties affect the efficiency of the coupling reactions in solid-phase peptide synthesis. Here we report a novel approach to evaluate resin solvation properties, making use of spin label electron ...