Buscar
Mostrando ítems 1-10 de 68
Structural basis for the broad specificity of a new family of amino-acid racemases
(Wiley Blackwell Publishing, Inc, 2014-01)
Broad-spectrum amino-acid racemases (Bsrs) enable bacteria to generate noncanonical D-amino acids, the roles of which in microbial physiology, including the modulation of cell-wall structure and the dissolution of biofilms, ...
A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids
(Elsevier Ltd, 2020-10)
The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, ...
Orthogonal Translation Meets Electron Transfer: In Vivo Labeling of Cytochrome c for Probing Local Electric Fields
(Wiley, 2015-02)
Cytochrome c (cyt c), a redox protein involved in diverse fundamental biological processes, is among the most traditional model proteins for analyzing biological electron transfer and protein dynamics both in solution and ...
Conformational dynamics in TRPV1 channels reported by an encoded coumarin amino acid
(2017)
TRPV1 channels support the detection of noxious and nociceptive input. Currently available functional and structural data suggest that TRPV1 channels have two gates within their permeation pathway: one formed by a ...
Machine learning reveals a non‐canonical mode of peptide binding to MHC class II molecules
(Wiley Blackwell Publishing, Inc, 2017-10)
MHC class II molecules play a fundamental role in the cellular immune system: they load short peptide fragments derived from extracellular proteins and present them on the cell surface. It is currently thought that the ...
Mapping the physiological response of oenococcus oeni to ethanol stress using an extended genome- scale metabolic model
(Frontiers Media, 2018)
The effect of ethanol on the metabolism of Oenococcus oeni, the bacterium responsible for the malolactic fermentation (MLF) of wine, is still scarcely understood. Here, we characterized the global metabolic response in O. ...
On the efficient bio-incorporation of 5-hydroxy-tryptophan in recombinant proteins expressed in Escherichia coli with T7 RNA polymerase-based vectors
(Academic Press Inc Elsevier Science, 2017)
Biosynthetic incorporation of non-canonic amino acids is an attractive strategy to introduce new properties in recombinant proteins. Trp analogs can be incorporated in recombinant proteins replacing regular Trp during ...
Mapping the physiological response of Oenococcus oeni to ethanol stress using an extended genome-scale metabolic model
(Frontiers Media S.A., 2018)
© 2018 Contreras, Ribbeck, Gutiérrez, Cañon, Mendoza and Agosin. The effect of ethanol on the metabolism of Oenococcus oeni, the bacterium responsible for the malolactic fermentation (MLF) of wine, is still scarcely ...
Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology
(Wiley-Blackwell, 2016)
Transfer RNAs (tRNAs) are the macromolecules that transfer activated amino acids from aminoacyl-tRNA synthetases to the ribosome, where they are used for the mRNA guided synthesis of proteins. Transfer RNAs are ancient ...
The canonical DHHC motif is not absolutely required for the activity of the yeast S-acyltransferases Swf1 and Pfa4
(American Society for Biochemistry and Molecular Biology, 2015-09)
Protein S-acyltransferases, also known as palmitoyltransferases (PATs), are characterized by the presence of a 50-amino acid domain called the DHHC domain. Within this domain, these four amino acids constitute a highly ...