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Expressão, purificação e caracterização bioquímica da mutante subtilisina-símile de natrialba magadii (nepct)
(Universidade Federal de São Paulo (UNIFESP), 2014-01-29)
Protótipo de halofilismo, Natrialba magadii foi isolada em um lago salino do '1i leste africano, crescendo em condições hipersalinas de pH 9,5 com 3,5M de NaCI e cujo genoma se encontra completamente sequenciado pelo Joint ...
Functional roles of C-terminal extension (CTE) of salt-dependent peptidase activity of the Natrialba magadii extracellular protease (NEP)
(Elsevier Science, 2018-07-07)
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many extracellular proteases have been characterized from archaea to bacteria as ...
The Lon protease from the haloalkaliphilic archaeon Natrialba magadii is transcriptionally linked to a cluster of putative membrane proteases and displays DNA-binding activity
(Elsevier Gmbh, 2011-05)
The ATP-dependent Lon protease is universally distributed in bacteria, eukaryotic organelles and archaea. In comparison with bacterial and eukaryal Lon proteases, the biology of the archaeal Lon has been studied to a limited ...
Growth phase-dependent biosynthesis of Nep, a halolysinlike protease secreted by the alkaliphilic haloarchaeon Natrialba magadii
(Wiley Blackwell Publishing, Inc, 2010-06-09)
Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin-like
protease (Nep) that is active and stable in high salt and in organic solvents,
which represents a potential resource for biocatalysis in low ...
Nmag_2608, an extracellular ubiquitin-like domain-containing protein from the haloalkaliphilic archaeon Natrialba magadii
(Springer Tokyo, 2012-05)
Ubiquitin-like proteins (Ubls) and ubiquitin-like domain-containing proteins (Ulds) found in both eukaryotes and prokaryotes display an ubiquitin fold. We previously characterized a 124-amino acid polypeptide (P400) from ...
Secondary structure determination by FTIR of an archaeal ubiquitin-like polypeptide from Natrialba magadii
(Springer, 2011-09)
The ubiquitin protein belongs to the β-grasp fold family, characterized by four or five β-sheets with a single α-helical middle region. Ubiquitin-like proteins (Ubls) are structural homologues with low sequence identity ...
Gene cloning and heterologous synthesis of a haloalkaliphilic extracellular protease of Natrialba magadii (Nep)
(Springer Tokyo, 2008-09)
The gene encoding the protease Nep secreted by the haloalkaliphilic archaeon Natrialba magadii was cloned and sequenced. Upstream of the nep gene, a region related to haloarchaeal TATA-box and BRE-like consensus sequences ...
Identification of growth-dependent transcripts in the haloalkaliphilic archaeon Natrialba magadii
(Applied Microbiology, Molecular and Cellular Biosciences Research Foundation, 2012-03)
The transition from balanced growth to the stationary phase induces changes in the morphology and physiology of most bacteria with the aim of preparing the cells to withstand unfavorable conditions (Nystrom, 2004; Siegele ...
Presence of structural homologs of ubiquitin in haloalkaliphilic Archaea
(Springer, 2009-09)
Ubiquitin, a protein widely conserved in eukaryotes, is involved in many cellular processes, including proteolysis. While sequences encoding ubiquitin-like proteins have not been identified in prokaryotic genomes sequenced ...
Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
(Wiley VCH Verlag, 2018-07)
The dynamic changes that take place along the phases of microbial growth (lag, exponential, stationary, and death) have been widely studied in bacteria at the molecular and cellular levels, but little is known for archaea. ...